CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF BETA-CRYPTOGEIN, A TOXIC ELICITIN SECRETED BY THE PHYTOPATHOGENIC FUNGUS PHYTOPHTHORA-CRYPTOGEA

被引：9

作者：

GUILLOTEAU, JP

NESPOULOUS, C

HUET, JC

BEAUVAIS, F

PERNOLLET, JC

BRUNIE, S

机构：

[1] INRA,UNITE INGN PROT,BATIMENT BIOTECHNOL,F-78352 JOUY EN JOSAS,FRANCE

[2] INRA,ETUD PROT LAB,F-78026 VERSAILLES,FRANCE

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 229卷 / 02期

关键词：

ELICITIN; PHYTOPHTHORA-CRYPTOGEA; PROTEIN CRYSTALLOGRAPHY;

D O I：

10.1006/jmbi.1993.1058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of the basic elicitin secreted by Phytophthora cryptogea have been obtained by the hanging-drop method of vapor diffusion from sodium chloride solutions. The crystals belong to the tetragonal space group P4122 (or enantiomorph P4322), with unit cell dimensions a = b = 47 Å, c = 137 Å and probably contain two molecules per asymmetric unit. The crystals are very stable to X-rays and diffract to 2.2 Å resolution on a synchrotron radiation source.

引用

页码：564 / 565

页数：2

共 8 条

[1]

COHN EJ, 1948, PROTEINS AMINO ACIDS

[2] STRUCTURES OF ELICITIN ISOFORMS SECRETED BY PHYTOPHTHORA-DRECHSLERI [J].