PHOTOCHROME-LABELING METHOD IN STUDY OF DYNAMICS OF BIOLOGICAL-SYSTEMS

被引:14
作者
LIKHTENSHTEIN, GI [1 ]
KHUDJAKOV, DV [1 ]
VOGEL, VR [1 ]
机构
[1] USSR ACAD SCI,INST CHEM PHYS,CHERNOGOLOVKA,USSR
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1992年 / 25卷 / 04期
关键词
PHOTOCHROME LABEL; PROTEIN DYNAMICS; MICROVISCOSITY; MYOGLOBIN;
D O I
10.1016/0165-022X(92)90016-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The theoretical considerations and experimental evidences discussed in this paper indicate that quantitative study of photochromic processes in labeled objects open up new possibilities for investigating microviscosity and conformation transitions in biological systems. The proposed method of photochrome labeling features higher sensitivity and simplicity, and uses labels which are more stable under physiological conditions compared with traditional spin labels.
引用
收藏
页码:219 / 229
页数:11
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