EXTRACELLULAR CA2(+)-DEPENDENT AND INDEPENDENT CALCIUM TRANSIENT IN FETAL MYOTUBES

被引:9
作者
STRUBE, C
BEURG, M
GEORGESCAULD, D
BOURNAUD, R
SHIMAHARA, T
机构
[1] LAB NEUROBIOL CELLULAIRE & MOLEC,CNRS,F-91198 GIF SUR YVETTE,FRANCE
[2] CTR RECH PAUL PASCAL,CNRS,F-33600 PESSAC,FRANCE
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1994年 / 427卷 / 5-6期
关键词
CALCIUM RELEASE; DIHYDROPYRIDINE RECEPTOR; MYOGENESIS; FURA-2; SKELETAL MUSCLE;
D O I
10.1007/BF00374269
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Spatio-temporal changes in the intracellular calcium concentration [Ca2+](i) of dissociated mice myotubes from 14-day and 18-day-old fetuses were studied using digital imaging analysis of the Ca2+ indicator fura-2. Myotubes from 18-day-old fetuses displayed a transient [Ca2+](i) increase upon electrical stimulation either in nominally calcium-free external solution or in Krebs solution containing 100 mu M lanthanum. Thus, at this developmental stage, membrane depolarization appears to increase [Ca2+](i) by stimulating Ca2+ release from the sarcoplasmic reticulum independently of extracellular Ca2+ influx. Similarly, myotubes from 14-day-old fetuses also showed a calcium transient upon electrical stimulation in Krebs solution. However, in 46% of these myotubes the calcium transient was abolished when Ca2+ entry through calcium channels was suppressed.
引用
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页码:517 / 523
页数:7
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