ROLE OF BACTERIAL AND PHAGE RECOMBINATION SYSTEMS AND OF DNA-REPLICATION IN GENETIC-RECOMBINATION OF UV-IRRADIATED PHAGE-LAMBDA

被引:1
作者
BLANCO, M [1 ]
ARMENGOD, ME [1 ]
机构
[1] INST INVEST CITOLOGICAS, GENET MICROBIANA LAB, VALENCIA 10, SPAIN
来源
MOLECULAR AND GENERAL GENETICS | 1976年 / 146卷 / 01期
关键词
D O I
10.1007/BF00267982
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The influence of the bacterial Rec and phage .lambda. Red recombination systems on the rescue of the O+ gene from the prophage by a superinfection UV irradiated or non-irradiated O- phage is studied in Escherichia coli K12. Without UV irradiation the Red system produces more recombinants than does the Rec system, and its action requires DNA replication. The presence of UV lesions in the .lambda.DNA facilitates the action of the Rec system, which is more efficient in this case than the Red system and can act without DNA replication. In all cases, there is a cooperation between the 2 generalized recombination systems.
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页码:51 / 54
页数:4
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