VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC RADIOIMMUNOASSAY METHOD FOR THE DETERMINATION OF LACIDIPINE IN PLASMA

被引：16

作者：

PELLEGATTI, M ^{[1
]}

BRAGGIO, S ^{[1
]}

SARTORI, S ^{[1
]}

FRANCESCHETTI, F ^{[1
]}

BOLELLI, GF ^{[1
]}

机构：

[1] UNIV BOLOGNA,CNR,REPROD ENDOCRINE UNIT,I-40126 BOLOGNA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80481-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and reproducible method for the determination of lacidipine, a new potent antihypertensive dihydropyridine, is reported. The method involves solid-phase extraction, reversed-phase high-performance liquid chromatography and radioimmunoassay of the collected fraction. The assay provides a limit of detection of 20 pg/ml of plasma, allowing the determination of trough (24 h) plasma concentrations. The method is suitable for pharmacokinetic studies in man.

引用

页码：105 / 111

页数：7

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