SUBSTRATE-SPECIFICITY OF ESCHERICHIA-COLI LD-CARBOXYPEPTIDASE ON BIOSYNTHETICALLY MODIFIED MUROPEPTIDES

被引：13

作者：

LEGUINA, JI ^{[1
]}

QUINTELA, JC ^{[1
]}

DEPEDRO, MA ^{[1
]}

机构：

[1] UAM, FAC CIENCIAS, CSIC, CTR BIOL MOLEC SEVERO OCHOA, E-28049 MADRID, SPAIN

来源：

FEBS LETTERS | 1994年 / 339卷 / 03期

关键词：

ESCHERICHIA COLI; CARBOXYPEPTIDASE; MUREIN; PEPTIDOGLYCAN; D-AMINO ACID;

D O I：

10.1016/0014-5793(94)80425-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli murein can be biosynthetically modified. Amino acids at positions 3 and 4 (m-diaminopimelic acid and D-alanine, respectively) on the peptide moieties can be changed under appropriate growth conditions. The activity of E. coli LD-carboxypeptidase on biosynthetically modified substrates has been studied in vitro. The enzyme hydrolysed all tested disaccharide-tetrapeptide monomeric muropeptides modified al position 4. Monomers with m-lanthionine, but not with L-ornithine, instead of m-diaminopimelic acid at position 3 were accepted. However, neither cross-linked muropeptides nor macromolecular murein were substrates for the reaction. Our observations argue against a direct effect of LD-carboxypeptidase on macromolecular murein metabolism.

引用

页码：249 / 252

页数：4

共 25 条

[1] ACTIVITY OF 3 MUREIN HYDROLASES DURING CELL-DIVISION CYCLE OF ESCHERICHIA-COLI K-12 AS MEASURED IN TOLUENE-TREATED CELLS [J].

BECK, BD ;

PARK, JT .

JOURNAL OF BACTERIOLOGY, 1976, 126 (03) :1250-1260

[2] THE BALANCE BETWEEN DIFFERENT PEPTIDOGLYCAN PRECURSORS DETERMINES WHETHER ESCHERICHIA-COLI-CELLS WILL ELONGATE OR DIVIDE [J].

BEGG, KJ ;

TAKASUGA, A ;

EDWARDS, DH ;

DEWAR, SJ ;

SPRATT, BG ;

ADACHI, H ;

OHTA, T ;

MATSUZAWA, H ;

DONACHIE, WD .

JOURNAL OF BACTERIOLOGY, 1990, 172 (12) :6697-6703

[3]

CAPARROS M, 1992, J BACTERIOL, V174, P5549

[4]

CAPARROS M, 1993, FEMS SYMP, P147

[5] MOLECULAR WEIGHT-DETERMINATION OF BIOSYNTHETICALLY MODIFIED MONOMERIC AND OLIGOMERIC MUROPEPTIDES FROM ESCHERICHIA-COLI BY PLASMA DESORPTION MASS-SPECTROMETRY [J].

CAPARROS, M ;

PITTENAUER, E ;

SCHMID, ER ;

DEPEDRO, MA ;

ALLMAIER, G .

FEBS LETTERS, 1993, 316 (02) :181-185

[6] GROWTH AND CELL-DIVISION OF ESCHERICHIA-COLI 173-25 IN PRESENCE OF SOME ANALOGS OF DIAMINOPIMELIC ACID [J].

CHALOUPKA, J ;

STRNADOVA, M ;

CASLAVSKA, J ;

VERES, K .

ZEITSCHRIFT FUR ALLGEMEINE MIKROBIOLOGIE, 1974, 14 (04) :283-296

[7]

GLAUNER B, 1990, J BIOL CHEM, V265, P18988

[8]

GLAUNER B, 1988, J BIOL CHEM, V263, P10088

[9] RECYCLING OF MUREIN BY ESCHERICHIA-COLI [J].

GOODELL, EW .

JOURNAL OF BACTERIOLOGY, 1985, 163 (01) :305-310

[10] TARGETS OF PENICILLIN ACTION IN ESCHERICHIA-COLI [J].

HARTMANN, R ;

HOLTJE, JV ;

SCHWARZ, U .

NATURE, 1972, 235 (5339) :426-&

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