LACK OF INVOLVEMENT OF T-LYMPHOCYTES IN THE LEUKEMIC POPULATION DURING PROLONGED CHRONIC PHASE OF PHILADELPHIA-CHROMOSOME-POSITIVE CHRONIC MYELOID-LEUKEMIA

被引：4

作者：

ARIAD, S ^{[1
]}

DAJEE, D ^{[1
]}

WILLEM, P ^{[1
]}

BEZWODA, WR ^{[1
]}

机构：

[1] UNIV WITWATERSRAND,DEPT MED HEMATOL ONCOL,JOHANNESBURG 2001,SOUTH AFRICA

来源：

LEUKEMIA & LYMPHOMA | 1993年 / 10卷 / 03期

关键词：

T-LYMPHOCYTES; LEUKEMIA; CHRONIC PHASE OF CML; PHILADELPHIA CHROMOSOME; CHRONIC MYELOID LEUKEMIA;

D O I：

10.3109/10428199309145886

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Nine patients with prolonged (>2 years) chronic phase chronic myeloid leukaemia (CML) were investigated for the presence of T-cell involvement in the leukemic clone. Pure populations of peripheral blood T-cell populations were obtained by culturing separated mononuclear cells in the presence of pokeweed mitogen and IL2, until cultures showed >99% pure T-cells. Purified T-cells and bone marrow and peripheral blood hematopoetic precursors were analysed for the presence of bcr-abl mRNA transcripts following RNA extraction and message amplification using polymerase chain reaction. In none of the 9 patients was bcr-abl mRNA found in T-lymphocytes while in all cases such transcripts were found in bone marrow and peripheral blood hematopoetic cells. Failure to detect T-cell involvement in patients with prolonged chronic phase CML using techniques designed to enhance even low level involvement of these cell populations supports the view that aquisition of the Ph chromosome abnormality does not occur in the totipotent stem cells but in more committed precursor cell/s with multilineage capacity but which only rarely retain the capacity for T-cell differentiation.

引用

页码：217 / 221

页数：5

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