CHARACTERIZATION OF THE MNP2 GENE ENCODING MANGANESE PEROXIDASE ISOZYME-2 FROM THE BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM

被引：46

作者：

MAYFIELD, MB ^{[1
]}

GODFREY, BJ ^{[1
]}

GOLD, MH ^{[1
]}

机构：

[1] OREGAN GRAD INST SCI & TECHNOL, DEPT CHEM BIOCHEM & MOLEC BIOL, PORTLAND, OR 97291 USA

来源：

GENE | 1994年 / 142卷 / 02期

基金：

美国国家科学基金会;

关键词：

LIGNIN BIODEGRADATION; GENOMIC CLONE; HEAT-SHOCK ELEMENT; SECONDARY METABOLISM; METAL RESPONSE ELEMENT; INTRONS;

D O I：

10.1016/0378-1119(94)90266-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide (nt) sequence of a gene (mnp2) encoding manganese peroxidase isozyme 2 (MnP-2) from Phanerochaete chrysosporium was determined. The sequence of 3297 bp includes 1287 bp of 5'-flanking sequence and 490 bp 3' to the stop codon. Comparison of cDNA and genomic sequences indicates seven introns varying in size from 50-55 bp. The 5' upstream region of the mnp2 gene contains a TATAA element, three inverted CCAAT elements (ATTGG), six putative heat-shock elements (HSE) and three putative metal response elements (MRE) (TGCRCNC), all located within 1100-bp upstream from the start codon. The positions of the putative HSE and MRE in the promoter region of mnp2 are compared with the corresponding sequences in the mnp1 gene promoter. A Northern blot probed with a fragment specific for the mnp2 gene suggests that the transcription of mnp2 is regulated by Mn ions.

引用

页码：231 / 235

页数：5

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