AMPEROMETRIC SENSOR FOR CHOLINE AND ACETYLCHOLINE BASED ON A PLATINUM-ELECTRODE MODIFIED BY A CO-CROSS-LINKED BIENZYMIC SYSTEM

被引：43

作者：

GUERRIERI, A ^{[1
]}

DEBENEDETTO, GE ^{[1
]}

PALMISANO, F ^{[1
]}

ZAMBONIN, PG ^{[1
]}

机构：

[1] UNIV BARI,DIPARTMENTO CHIM,I-70126 BARI,ITALY

来源：

ANALYST | 1995年 / 120卷 / 11期

关键词：

BIOSENSOR; CHOLINE; ACETYLCHOLINE;

D O I：

10.1039/an9952002731

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast-response and sensitive amperometric biosensor for choline and acetylcholine based on choline oxidase and acetylcholinesterase immobilized on a platinum electrode by co-crosslinking with bovine serum albumin and glutaraldehyde has been developed. The immobilization procedure proved fast and simple, not requiring dedicated manipulation. Detection limits mere in the sub-micromolar range and linearity extended over three concentration decades. After one month of discontinuous use, 70% of the initial sensitivity was still observed. The response time was around 1 s for both analytes making the developed biosensor a suitable detector for flow injection systems and high-performance liquid chromatography.

引用

页码：2731 / 2736

页数：6

共 36 条

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