A NEW PURIFICATION PROCEDURE FOR CLOSTRIDIUM-DIFFICILE ENTERO-TOXIN

被引：28

作者：

RIHN, B ^{[1
]}

SCHEFTEL, JM ^{[1
]}

GIRARDOT, R ^{[1
]}

MONTEIL, H ^{[1
]}

机构：

[1] UNIV STRASBOURG 1, FAC MED, INST BACTERIOL, F-67070 STRASBOURG, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1984年 / 124卷 / 03期

关键词：

D O I：

10.1016/0006-291X(84)91013-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C. difficile produces 2 toxins, an enterotoxin and a cytotoxin. The enterotoxin was purified using fast methods (tangential flow filtration, fast protein liquid chromatography). The purified enterotoxin is composed of 2 subunits (A1 = 41,500 MW, A2 = 16,000 MW) and its isoelectric point is 3.5.

引用

页码：690 / 695

页数：6

共 15 条

[1] GEL-FILTRATION BEHAVIOUR OF PROTEINS RELATED TO THEIR MOLECULAR WEIGHTS OVER A WIDE RANGE [J].

ANDREWS, P .

BIOCHEMICAL JOURNAL, 1965, 96 (03) :595-&

[2]

[Anonymous], 1982, ISOELECTRIC FOCUSING

[3]

BANNO Y, 1981, BIOCHEM INT, V2, P629

[4]

BANNO Y, 1984, REV INFECT DIS, V6, pS11

[5] EFFECTS OF THE 2 TOXINS OF CLOSTRIDIUM-DIFFICILE IN ANTIBIOTIC-ASSOCIATED CECITIS IN HAMSTERS [J].

LIBBY, JM ;

JORTNER, BS ;

WILKINS, TD .

INFECTION AND IMMUNITY, 1982, 36 (02) :822-829

[6]

LONNROTH I, 1983, ACTA PATH MICRO IM B, V91, P395

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8]

MAIZEL JV, 1970, NATURE, V227, P680

[9]

PIERCE NF, 1972, GASTROENTEROLOGY, V63, P439

[10] PURIFICATION AND CHARACTERIZATION OF CLOSTRIDIUM-DIFFICILE TOXIN [J].

ROLFE, RD ;

FINEGOLD, SM .

INFECTION AND IMMUNITY, 1979, 25 (01) :191-201

← 1 2 →