EFFECT OF PH SHIFTS ON RADIOSENSITIVITY OF HUMAN-LYMPHOCYTES IRRADIATED IN THE G2 STAGE

被引：7

作者：

BOSI, A ^{[1
]}

MICHELI, A ^{[1
]}

PIETROSANTI, S ^{[1
]}

OLIVIERI, G ^{[1
]}

机构：

[1] UNIV ROME LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOLEC,P ALDO MORO 5,I-00185 ROME,ITALY

来源：

MUTATION RESEARCH | 1991年 / 250卷 / 1-2期

关键词：

PH; CHROMATID ABERRATIONS; HUMAN LYMPHOCYTES; X-RAYS;

D O I：

10.1016/0027-5107(91)90188-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Experiments were carried out using human lymphocytes in order to test the effect of pH shifts on radiosensitivitY of cells irradiated in the G2 Stage. In our culture conditions constant variations in medium pH over the range of 7.4-6.8 were observed as a function of incubation time after PHA stimulation; in addition the pH of the medium was adjusted in parallel cultures over the range of 5.9-8.2. Then we exposed the cultures with different pH to a treatment of X-rays delivered 2 h before fixation. The pH of the medium was found to greatly affect the yield of induced chromatid aberrations.

引用

页码：325 / 329

页数：5

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