ACUTE OSMOTIC TOLERANCE OF CULTURED-CELLS OF THE OYSTER PATHOGEN PERKINSUS-MARINUS (APICOMPLEXA, PERKINSIDA)

被引：30

作者：

BURRESON, EM ^{[1
]}

CALVO, LMR ^{[1
]}

LAPEYRE, JF ^{[1
]}

COUNTS, F ^{[1
]}

PAYNTER, KT ^{[1
]}

机构：

[1] UNIV MARYLAND, DEPT ZOOL, COLLEGE PK, MD 20742 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 1994年 / 109卷 / 03期

关键词：

CULTURED CELLS; OSMOTIC TOLERANCE; OYSTER PATHOGEN; PERKINSUS MARINUS;

D O I：

10.1016/0300-9629(94)90196-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cultured Perkinsus marinus cells were exposed for 24 hr to salinities of 0, 3, 6, 9, 12 and 22 ppt at temperatures of 1, 5, 10, 15 and 28 degrees C in artificial seawater (ASW) and to the same salinities at 28 degrees C in ASW with the osmotic concentration adjusted with sucrose to the equivalent of 22ppt. At 28 degrees C mortality increased as salinity decreased below 22ppt. Mortality was greater than 99% at 0 ppt and greater than 90% at 3 ppt. Mortality was 70% at 6 ppt, 43% at 9 ppt and 20% at 12 ppt. Mortality was low (<5%) and equal to that at 22 ppt in all treatments where osmotic concentration was maintained with sucrose. Mortality occurred rapidly, within 5 min of exposure to experimental conditions. In the region where mortality was most sensitive to salinity changes (6-12 ppt), lower temperature caused an increase in mortality, but the temperature effect was significant only at 9 ppt.

引用

页码：575 / 582

页数：8

共 32 条

[1] SALINITY RESPONSES OF THE MARINE MICROALGA BRACHIOMONAS-SUBMARINA BOHLIN [J].