MOLECULAR-CLONING AND NUCLEOTIDE SEQUENCING OF THE 3'-TERMINAL GENOMIC RNA OF THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS

被引:134
作者
MENG, XJ
PAUL, PS
HALBUR, PG
机构
[1] IOWA STATE UNIV SCI & TECHNOL,COLL VET MED,VET MED RES INST,AMES,IA 50011
[2] IOWA STATE UNIV SCI & TECHNOL,COLL VET MED,DEPT MICROBIOL IMMUNOL & PREVENT MED,AMES,IA 50011
关键词
D O I
10.1099/0022-1317-75-7-1795
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The genomic RNA of a porcine reproductive and respiratory syndrome virus (PRRSV) isolate from the U.S.A., VR 2385 (ATCC), was copied into cDNA after priming with oligo(dT) and cloned into phage lambda. The cDNA clones representing the 3'-terminal genomic RNA of the virus were isolated and sequenced. The genome is a positive-stranded, polyadenylated RNA with an estimated size of 15 kb. Analysis of the resulting sequence identified three complete open reading frames (ORFs) with the potential to encode polypeptides with predicted M(r)s of 22.2K (ORF 5), 19.1K (ORF 6) and 13.6K (ORF 7). ORF 7, which is closest to the 3' end, is predicted to encode a highly basic nucleocapsid protein displaying 58 % amino acid identity to the corresponding protein of the Lelystad virus (LV), a European PRRSV isolate. ORFs 6 and 5, preceding ORF 7, are each predicted to encode proteins containing several hydrophobic domains that are thought to be membrane-associated. The VR 2385 ORF 6 protein is the most conserved structural protein. It has 78% amino acid identity to the equivalent LV protein, and ORF 5 shares only 54% of its amino acid sequence. Northern blot analysis revealed a 3'-coterminal nested set of six subgenomic RNAs in VR 2385 virus-infected CRL 11171 cells. Our results indicate that VR 2385, like LV, is a member of the newly proposed arterivirus group. However, the striking genetic variation and the difference in pathogenicity between LV and VR 2385 suggest that the viruses causing PRRS in the U.S.A. and Europe are highly variable and they may represent different genotypes.
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页码:1795 / 1801
页数:7
相关论文
共 30 条
  • [1] COMPARISON OF PORCINE ALVEOLAR MACROPHAGES AND CL-2621 FOR THE DETECTION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUS AND ANTI-PRRS ANTIBODY
    BAUTISTA, EM
    GOYAL, SM
    YOON, IJ
    JOO, HS
    COLLINS, JE
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (02) : 163 - 165
  • [2] CHARACTERIZATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME (SIRS) VIRUS (ISOLATE ATCC VR-2332)
    BENFIELD, DA
    NELSON, E
    COLLINS, JE
    HARRIS, L
    GOYAL, SM
    ROBISON, D
    CHRISTIANSON, WT
    MORRISON, RB
    GORCYCA, D
    CHLADEK, D
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (02) : 127 - 133
  • [3] SEQUENCES OF 3' END OF GENOME AND OF 5' END OF OPEN READING FRAME 1A OF LACTATE DEHYDROGENASE-ELEVATING VIRUS AND COMMON JUNCTION MOTIFS BETWEEN 5' LEADER AND BODIES OF 7 SUBGENOMIC MESSENGER-RNAS
    CHEN, ZY
    KUO, L
    ROWLAND, RRR
    EVEN, C
    FAABERG, KS
    PLAGEMANN, PGW
    [J]. JOURNAL OF GENERAL VIROLOGY, 1993, 74 : 643 - 660
  • [4] CHRISTIANSON WT, 1992, AM J VET RES, V53, P485
  • [5] ISOLATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME VIRUS (ISOLATE ATCC VR-2332) IN NORTH-AMERICA AND EXPERIMENTAL REPRODUCTION OF THE DISEASE IN GNOTOBIOTIC PIGS
    COLLINS, JE
    BENFIELD, DA
    CHRISTIANSON, WT
    HARRIS, L
    HENNINGS, JC
    SHAW, DP
    GOYAL, SM
    MCCULLOUGH, S
    MORRISON, RB
    JOO, HS
    GORCYCA, D
    CHLADEK, D
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (02) : 117 - 126
  • [6] MOLECULAR CHARACTERIZATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS, A MEMBER OF THE ARTERIVIRUS GROUP
    CONZELMANN, KK
    VISSER, N
    VANWOENSEL, P
    THIEL, HJ
    [J]. VIROLOGY, 1993, 193 (01) : 329 - 339
  • [7] PRRS SYNDROME IN QUEBEC - ISOLATION OF A VIRUS SEROLOGICALLY RELATED TO LELYSTAD VIRUS
    DEA, S
    BILODEAU, R
    ATHANASEOUS, R
    SAUVAGEAU, RA
    MARTINEAU, GP
    [J]. VETERINARY RECORD, 1992, 130 (08) : 167 - 167
  • [8] EQUINE ARTERITIS VIRUS IS NOT A TOGAVIRUS BUT BELONGS TO THE CORONAVIRUSLIKE SUPERFAMILY
    DENBOON, JA
    SNIJDER, EJ
    CHIRNSIDE, ED
    DEVRIES, AAF
    HORZINEK, MC
    SPAAN, WJM
    [J]. JOURNAL OF VIROLOGY, 1991, 65 (06) : 2910 - 2920
  • [9] ALL SUBGENOMIC MESSENGER-RNAS OF EQUINE ARTERITIS VIRUS CONTAIN A COMMON LEADER SEQUENCE
    DEVRIES, AAF
    CHIRNSIDE, ED
    BREDENBEEK, PJ
    GRAVESTEIN, LA
    HORZINEK, MC
    SPAAN, WJM
    [J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (11) : 3241 - 3247
  • [10] COMPLETE GENOMIC SEQUENCE AND PHYLOGENETIC ANALYSIS OF THE LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV)
    GODENY, EK
    CHEN, L
    KUMAR, SN
    METHVEN, SL
    KOONIN, EV
    BRINTON, MA
    [J]. VIROLOGY, 1993, 194 (02) : 585 - 596