CALCIUM OSCILLATIONS INDEX THE EXTENT OF CALCIUM LOADING AND PREDICT FUNCTIONAL RECOVERY DURING REPERFUSION IN RAT MYOCARDIUM

被引:23
作者
WEISS, RG
GERSTENBLITH, G
LAKATTA, EG
机构
[1] NIA,GERONTOL RES CTR,CARDIOVASC SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224
[2] JOHNS HOPKINS UNIV HOSP,DEPT MED,DIV CARDIOL,BALTIMORE,MD 21205
关键词
Diastolic calcium oscillations; High-energy phosphates; Reperfusion;
D O I
10.1172/JCI114501
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Delayed recovery of contractile function after myocardial ischemia may be due to prolonged recovery of high-energy phosphates, persistent acidosis, increased inorganic phosphate, and/or calcium loading. To examine these potential mechanisms, metabolic parameters measured by 31P nuclear magnetic resonance spectroscopy, and spontaneous diastolic myofilament motion caused by sarcoplasmic reticulum-myofilament calcium cycling indexed by the scattered light intensity fluctuations (SLIF) it produces in laser beam reflected from the heart, were stuided in isolated atrioventricularly blocked rat hearts (n = 10) after 65 min of ischemia at 30°C. All metabolic parameters recovered to their full extent 5 min after reperfusion. Developed pressure evidenced a small recovery but then fell abruptly. This was accompanied by an increase in end diastolic pressure to 37 ± 5 mm Hg and a fourfold increase in SLIF, to 252 ± 58% of baseline. In another series of hearts initial reperfusion with calcium of 0.08 mM prevented the SLIF rise and resulted in improved developed pressure (74 ± 3% vs. 39 ± 13% of control), and lower cell calcium (5.9 ± 3 vs. 10.3 ± 1.4 μmol/g dry wt). Thus, during reperfusion, delayed contractile recovery is not associated with delayed recovery of pH, inorganic phosphate, or high-energy phosphates and can be attributed, in part, to an adverse effect of calcium loading which can be indexed by increased SLIF occurring at that time.
引用
收藏
页码:757 / 765
页数:9
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