CIRCULARLY POLARIZED LUMINESCENCE AS A PROBE OF METAL-IONS BINDING-SITES IN CALMODULIN

被引：6

作者：

CORUH, N ^{[1
]}

RIEHL, JP ^{[1
]}

机构：

[1] UNIV MISSOURI,DEPT CHEM,ST LOUIS,MO 63121

来源：

COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS | 1991年 / 56卷 / 12期

关键词：

D O I：

10.1135/cccc19913028

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Circularly polarized luminescence (CPL) from dilute solutions of Tb(III) bound to the Ca-binding protein calmodulin is reported. The dissymmetry ratio, g(em), at 543.5 nm can be monitored as a function of equivalents of metal ion concentration. Competitive and consecutive addition of Ca(II) versus Tb(III) yield results which are consistent with previous results that suggest that Tb(III) and Ca(II) have a preferred affinity for different metal-ion binding sites in this protein.

引用

页码：3028 / 3031

页数：4

共 12 条

[1] A FLOW-DIALYSIS METHOD FOR OBTAINING RELATIVE MEASURES OF ASSOCIATION CONSTANTS IN CALMODULIN METAL-ION SYSTEMS [J].