EFFECTS OF TRANSFORMING GROWTH-FACTORS AND REGULATION OF THEIR MESSENGER-RNA LEVELS IN 2 HUMAN ENDOMETRIAL ADENOCARCINOMA CELL-LINES

被引:28
作者
ANZAI, Y
GONG, YW
HOLINKA, CF
MURPHY, LJ
MURPHY, LC
KURAMOTO, H
GURPIDE, E
机构
[1] CUNY MT SINAI SCH MED, DEPT OBSTET GYNECOL & REPROD SCI, NEW YORK, NY 10029 USA
[2] ORGANON INC, DEPT CLIN DEV, W ORANGE, NJ 07052 USA
[3] UNIV MANITOBA, DEPT PHYSIOL, WINNIPEG R3T 2N2, MANITOBA, CANADA
[4] KITASATO UNIV, DEPT OBSTET & GYNECOL, SAGAMIHARA, KANAGAWA 228, JAPAN
关键词
D O I
10.1016/0960-0760(92)90256-I
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of the transforming growth factor-beta-1 (TGF-beta-1) and epidermal growth factor (EGF) on the growth of cells from 2 endometrial cancer lines, Ishikawa and HEC-50 were evaluated by measuring rates of DNA synthesis and changes in cell numbers during culture. EGF at 17 and 1.7 nM concentrations consistently enhanced HEC-50 cell proliferation. TGF-beta-1 inhibited Ishikawa cell proliferation but, unexpectedly for epithelium-derived cells, stimulated HEC-50 cell growth. This effect is of interest as it indicates that endometrial cells can acquire an altered responsiveness to a growth inhibitor during the process of malignant transformation. Northern blot analyses showed expression of TGF-alpha, TGF-beta-1 and EGF receptors mRNA in both cell lines. Neither estradiol (E2) nor 4-hydroxytamoxifen (OHTam) affected mRNA levels for either TGF-alpha or TGF-beta in HEC-50 cells, a line unresponsive to E2 for proliferation. In Ishikawa cells, previously shown to respond to both E2 and OHTam by increasing proliferation rates, E2 increased TGF-alpha mRNA and reduced TGF-beta mRNA levels. OHTam lowered the levels of both mRNA species, although the effect was greater on TGF-beta than TGF-alpha mRNA. These data are consistent with, but do not prove, the existence of a possible autocrine regulation by TGF-alpha and TGF-beta of human cancer cell proliferation, which might be under E2 influence in Ishikawa cells.
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页码:449 / 455
页数:7
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