A SIMPLE TECHNIQUE FOR ONLINE MEASUREMENT OF CONTRACTIONS OF SINGLE SMOOTH-MUSCLE FIBERS UNDER CURRENT OR VOLTAGE-CLAMP

This paper describes a simple technique for routine on-line measurement of length and unloaded, isotonic contractions of single smooth muscle fibers during electrophysiological experiments. The fiber is held by the recording electrode itself, stretched straight in a fast-flowing stream of solution. The video image of the fiber is measured on-line by a simple computer program. Unlike other optical methods of tracking fiber length, the technique does not require a rigid one-dimensional contraction of the fiber. The technique is reliable and easy to use, and readily compatible with current clamp, voltage clamp, and rapid reversible application of neurotransmitters and drugs.