NUMERICAL-STUDIES OF MULTICOMPONENT CHROMATOGRAPHY USING PH GRADIENTS

被引:28
作者
FREY, DD
BARNES, A
STRONG, J
机构
[1] Dept. of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, Maryland
[2] Dept. of Mathematics, Duke University, Durham, North Carolina
关键词
D O I
10.1002/aic.690410513
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
A general numerical method is developed for multicomponent chromatography for the case where a pH gradient occurs and several buffering species are present that become adsorbed together with the components being separated through an ion-exchange mechanism. Acid-base equilibrium relations are used to determine the ionic compositions in the liquid and adsorbed phases and are solved using a single-variable Newton's method. The differential material-balance relations are integrated numerically using the method of characteristics. The transport relations are incorporated using a matrix analog of the linear-driving-force approximation. The resulting method accounts for the adsorption of each ionic form of each buffering species, for multicomponent diffusional interactions arising from induced electric fields, for volume and concentration overloading of proteins, and for changes in. the adsorption capacity caused by pH variations. Numerical calculations illustrate factors governing the selection of the adsorbent and buffer components for use in separating mixtures of proteins using retained, internally generated pH gradients.
引用
收藏
页码:1171 / 1183
页数:13
相关论文
共 28 条
[1]   METHOD OF CHARACTERISTICS TECHNIQUE - APPLICATION TO HEAT AND MASS TRANSFER PROBLEMS [J].
ACRIVOS, A .
INDUSTRIAL AND ENGINEERING CHEMISTRY, 1956, 48 (04) :703-710
[2]  
[Anonymous], 1986, NUMERICAL RECIPES
[3]   A VERSATILE MODEL FOR SIMULATION OF REACTION AND NONEQUILIBRIUM DYNAMICS IN MULTICOMPONENT FIXED-BED ADSORPTION PROCESSES [J].
BERNINGER, JA ;
WHITLEY, RD ;
ZHANG, X ;
WANG, NHL .
COMPUTERS & CHEMICAL ENGINEERING, 1991, 15 (11) :749-768
[4]  
BROOKS CA, 1992, AICHE J, V38, P1853
[5]   BUFFER-FACILITATED PROTON TRANSPORT - PH PROFILE OF BOUND ENZYMES [J].
ENGASSER, JM ;
HORVATH, C .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 358 (01) :178-192
[6]   GENERAL LINEARIZED MATRIX-THEORY OF ELECTROCHEMICAL MASS-TRANSFER WITH APPLICATIONS TO ELECTRODE PROCESSES AND ION-EXCHANGE [J].
FREY, DD ;
WONG, T .
CHEMICAL ENGINEERING COMMUNICATIONS, 1989, 75 :195-211
[7]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF AMINO-ACID PEPTIDES AND PROTEINS .83. EVALUATION OF RETENTION AND BANDWIDTH RELATIONSHIPS FOR PROTEINS SEPARATED BY ISOCRATIC ANION-EXCHANGE CHROMATOGRAPHY [J].
HEARN, MTW ;
HODDER, AN ;
STANTON, PG ;
AGUILAR, MI .
CHROMATOGRAPHIA, 1987, 24 :769-776
[8]  
Helfferich F.G., 1984, SOLVENT EXTR ION EXC, V2, P1151
[9]   WEAK ELECTROLYTES, POLYBASIC ACIDS, AND BUFFERS IN ANION-EXCHANGE COLUMNS .1. SODIUM-ACETATE AND SODIUM-CARBONATE SYSTEMS [J].
HELFFERICH, FG ;
BENNETT, BJ .
REACTIVE POLYMERS, 1984, 3 (01) :51-66
[10]   DEVELOPMENT OF FOCUSING BUFFER SYSTEMS FOR GENERATION OF WIDE-RANGE PH GRADIENTS DURING HIGH-PERFORMANCE CHROMATOFOCUSING [J].
HUTCHENS, TW ;
LI, CM ;
BESCH, PK .
JOURNAL OF CHROMATOGRAPHY, 1986, 359 :157-168