PROLIFERATIVE AND ANTIPROLIFERATIVE EFFECTS OF INTERFERON-GAMMA AND TUMOR-NECROSIS-FACTOR-ALPHA ON CELL-LINES DERIVED FROM CERVICAL AND OVARIAN MALIGNANCIES

被引:25
作者
MUTCH, DG
MASSAD, LS
KAO, MS
COLLINS, JL
机构
[1] Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Washington University School of Medicine St. Louis, MO
关键词
TUMOR NECROSIS FACTOR-ALPHA; INTERFERON-GAMMA; CELL GROWTH; GYNECOLOGIC MALIGNANCY;
D O I
10.1016/0002-9378(90)90774-2
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Four human cell lines derived from cervical carcinomas (ME-180, SiHa, HT-3, and MS751) and three human cell lines derived from ovarian carcinomas (SK-OV-3, Caov-3, and NIH:OVCAR-3) were analyzed in vitro to determine the effect of recombinant interferon-gamma and recombinant human tumor necrosis factor-alpha on cell growth and survival. The effects of interferon-gamma, tumor necrosis factor-alpha, and both interferon-gamma and tumor necrosis factor-alpha on cell growth were measured after 24 and 72 hours of incubation by the incorporation of chromium 51. The results of this analysis showed that all seven cell lines were resistant to the antiproliferative action of tumor necrosis factor-alpha, that the growth of most cell lines was inhibited by interferon-gamma by 72 hours of incubation, and that after 72 hours of incubation all cell lines demonstrated a synergistic antiproliferative response to the combination of interferon-gamma and tumor necrosis factor-alpha. However, the effects of these cytokines on cell growth were found to differ among cell lines and varied with the concentration and the duration of incubation. The growth of one cell lines (Caov-3) was stimulated by both tumor necrosis factor-alpha and interferon-gamma. These results suggest that the clinical effects of these cytokines on the growth of gynecologic cancers may be more complex than previously supposed.
引用
收藏
页码:1920 / 1924
页数:5
相关论文
共 23 条
[1]  
Fransen, van der Heyden, Ruysschaert, Fiers, Recombinant tumor necrosis factor: its effect and its synergism with interferon--y on a variety of normal and transformed human cell lines, Eur J Cancer Clin Oncol, 22, pp. 419-426, (1986)
[2]  
Sugarman, Aggarwal, Hass, Figari, Palladino, Shepard, Recombinant human tumor necrosis factor-a: effects on proliferation of normal and transformed cells in vitro, Science, 230, pp. 943-945, (1985)
[3]  
Mori, Kondoh, Tamaya, Synergistic effects of interferon-y and tumor necrosis factor against proliferation of gynecologic tumor cell lines, Gynecol Oncol, 35, pp. 209-214, (1989)
[4]  
Taguchi, Abe, Nakano, Matsui, Sohmura, Synergistic enhancement of the antitumor activity of recombinant human TNF-a by recombinant human IFN-y, Cancer Detect Prev, 12, pp. 105-114, (1988)
[5]  
Salmon, Young, Scuderi, Clark, Antineoplastic effects of tumor necrosis factor alpha alone and in combination with gamma-interferon on tumor biopsies in clonogenic assay, J Clin Oncol, 5, pp. 1816-1821, (1987)
[6]  
Jacobs, Dawoud, Kovacs, Ratliff, Inhibition of proliferation of lines derived from human cervical carcinomas by cytotoxic drugs and by recombinant interferons, Gynecol Oncol, 32, pp. 31-36, (1989)
[7]  
Spriggs, Imamura, Rodriguez, Sariban, Kufe, Tumor necrosis factor expression in human epithelial tumor cell lines, Journal of Clinical Investigation, 88, pp. 455-460, (1988)
[8]  
Marth, Helmberg, Mayer, Fuith, Daxenbichler, Dapunt, Effects of biological response modifiers on ovarian carcinoma cell lines, Anticancer Res, 9, pp. 461-468, (1989)
[9]  
Feinberg, Kurzrock, Talpaz, Blick, Saks, Gutterman, A phase I trial of intravenously-administered recombinant tumor necrosis factor-alpha in cancer patients, J Clin Oncol, 6, pp. 1328-1334, (1988)
[10]  
Chapman, Lester, Casper, Et al., Clinical pharmacology of recombinant human tumor necrosis factor in patients with advanced cancer, J Clin Oncol, 5, pp. 1942-1951, (1987)