MONENSIN AND FORSKOLIN INHIBIT THE TRANSCRIPTION RATE OF SUCRASE-ISOMALTASE BUT NOT THE STABILITY OF ITS MESSENGER-RNA IN CACO-2 CELLS

被引：14

作者：

CHANTRET, I

LACASA, M

CHEVALIER, G

SWALLOW, D

ROUSSET, M

机构：

[1] IRSC,VIRUS & DIFFERENCIAT LAB,F-94800 VILLEJUIF,FRANCE

[2] UNIV PARIS 06,F-75230 PARIS 05,FRANCE

[3] UNIV LONDON UNIV COLL,MRC,GALTON LAB,HUMAN BIOCHEM GENET UNIT,LONDON NW1 2HE,ENGLAND

来源：

FEBS LETTERS | 1993年 / 328卷 / 1-2期

关键词：

SUCRASE ISOMALTASE; CACO-2; CELL; FORSKOLIN; MONENSIN; TRANSCRIPTION; GLUCOSE REPRESSION;

D O I：

10.1016/0014-5793(93)80964-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of Caco-2 cells with forskolin (25 muM) or monensin (I muM) has previously been shown to cause a marked decrease in the level of sucrase-isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP-IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady-state level of SI mRNA both in forskolin- and monensin-treated Caco-2 cells.

引用

页码：55 / 58

页数：4

共 22 条

[1] POLY(A), POLY(A) BINDING-PROTEIN AND THE REGULATION OF MESSENGER-RNA STABILITY
BERNSTEIN, P
ROSS, J
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (09) : 373 - 377
[2] ON OFF REGULATION OF GENE-EXPRESSION AT THE LEVEL OF SPLICING
BINGHAM, PM
CHOU, TB
MIMS, I
ZACHAR, Z
[J]. TRENDS IN GENETICS, 1988, 4 (05) : 134 - 138
[3] GLUCOSE-REGULATED PROTEIN (GRP94 AND GRP78) GENES SHARE COMMON REGULATORY DOMAINS AND ARE COORDINATELY REGULATED BY COMMON TRANS-ACTING FACTORS
CHANG, SC
ERWIN, AE
LEE, AS
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (05) : 2153 - 2162
[4] SEQUENCE OF THE COMPLETE CDNA AND THE 5' STRUCTURE OF THE HUMAN SUCRASE-ISOMALTASE GENE - POSSIBLE HOMOLOGY WITH A YEAST GLUCOAMYLASE
CHANTRET, I
LACASA, M
CHEVALIER, G
RUF, J
ISLAM, I
MANTEI, N
EDWARDS, Y
SWALLOW, D
ROUSSET, M
[J]. BIOCHEMICAL JOURNAL, 1992, 285 : 915 - 923
[5] MONENSIN INHIBITS THE EXPRESSION OF SUCRASE-ISOMALTASE IN CACO-2 CELLS AT THE MESSENGER-RNA LEVEL
CHANTRET, I
TRUGNAN, G
DUSSAULX, E
ZWEIBAUM, A
ROUSSET, M
[J]. FEBS LETTERS, 1988, 235 (1-2) : 125 - 128
[6] ISOLATION OF A CDNA PROBE FOR THE HUMAN INTESTINAL DIPEPTIDYLPEPTIDASE-IV AND ASSIGNMENT OF THE GENE LOCUS DPP4 TO CHROMOSOME-2
DARMOUL, D
LACASA, M
CHANTRET, I
SWALLOW, DM
TRUGNAN, G
[J]. ANNALS OF HUMAN GENETICS, 1990, 54 : 191 - 197
[7] DECREASE OF MESSENGER-RNA LEVELS AND BIOSYNTHESIS OF SUCRASE-ISOMALTASE BUT NOT DIPEPTIDYLPEPTIDASE-IV IN FORSKOLIN OR MONENSIN-TREATED CACO-2 CELLS
DARMOUL, D
BARICAULT, L
SAPIN, C
CHANTRET, I
TRUGNAN, G
ROUSSET, M
[J]. EXPERIENTIA, 1991, 47 (11-12): : 1211 - 1215
[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
[9] ISOLATION OF A CDNA PROBE FOR A HUMAN JEJUNAL BRUSH-BORDER HYDROLASE, SUCRASE-ISOMALTASE, AND ASSIGNMENT OF THE GENE LOCUS TO CHROMOSOME-3
GREEN, F
EDWARDS, Y
HAURI, HP
POVEY, S
HO, MW
PINTO, M
SWALLOW, D
[J]. GENE, 1987, 57 (01) : 101 - 110
[10] IMMUNOGLOBULIN MESSENGER-RNA STABILITY VARIES DURING LYMPHOCYTE-B DIFFERENTIATION
JACK, HM
WABL, M
[J]. EMBO JOURNAL, 1988, 7 (04) : 1041 - 1046

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