PURIFICATION OF XYLANASE FROM TRICHODERMA-VIRIDE BY PRECIPITATION WITH AN ANIONIC POLYMER EUDRAGIT S-100

被引：51

作者：

GUPTA, MN

DONG, GQ

KAUL, R

MATTIASSON, B

机构：

[1] INDIAN INST TECHNOL, DEPT CHEM, NEW DELHI 110016, INDIA

[2] LUND UNIV, CTR CHEM, DEPT BIOTECHNOL, S-22100 LUND, SWEDEN

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 02期

关键词：

D O I：

10.1007/BF00152851

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Endo-xylanase from T. viride has been purified 4.2 fold by precipitation with a commercially available enteric polymer Eudragit S-100. Electrophoretic analysis also indicated removal of contaminant proteins. The enzyme could be recovered in more than 89% yield. The binding of the enzyme to the polymer was predominantly by electrostatic interaction.

引用

页码：117 / 122

页数：6

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