INTEGRATED APPLICATION OF CAPILLARY HPLC/CONTINUOUS-FLOW LIQUID SECONDARY-ION MASS-SPECTROMETRY TO DISCOVERY STAGE METABOLISM STUDIES

被引：13

作者：

LI, C

CHAURET, N

DUCHARME, Y

TRIMBLE, LA

NICOLLGRIFFITH, DA

YERGEY, JA

机构：

[1] Medicinal Chemistry Department, Merck Frosst Centre for Therapeutic Research, P.O. Box 1005, Pointe-Claire-Dorval

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 17期

关键词：

D O I：

10.1021/ac00113a030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of capillary HPLC/continuous-flow liquid secondary ion mass spectrometry (CF-LSIMS) as part of an integrated approach for characterizing discovery stage in vitro metabolites, using a specific inhibitor for 5-lipoxy-genase as a model compound, was investigated. CF-LSIMS demonstrated excellent sensitivity in detecting the metabolites in both the positive and the negative ion modes, with a good full-scan mass spectrum obtained when 5 pmol of metabolite was injected onto the capillary column. Strong pseudomolecular ions and key fragment ions were observed in the primary spectra of the parent drug and its three oxidative in vitro metabolites, allowing the site of metabolism to be pinpointed to particular substructures. This technique demonstrated versatility and offered a very rapid screening procedure for metabolite identification.

引用

页码：2931 / 2936

页数：6

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