CHARACTERIZATION OF GAP-JUNCTIONS BETWEEN PAIRS OF LEYDIG-CELLS FROM MOUSE TESTIS

Leydig cells are coupled in vivo by numerous gap junctions. In vivo and in vitro cells were immunolabeled by connexin 43 (Cx43) but not by Cx26 or Cx32 antibodies; immunoblotting confirmed specificity of Cx43 labeling. Pairs of Leydig cells dissociated from mouse testis were studied by dual whole cell voltage clamp, and a high incidence of dye (n = 20) and electrical coupling in = 60; >90%) was found. Coupling coefficients were near 1 and junctional conductance (g(j)) averaged 7.2 +/- 1.2 nS (SE, n = 40). Large transjunctional voltage (V-j) decreased g(j); currents decayed exponentially with time constants of seconds that decreased at greater V-j. The residual conductance at large V-j was at least similar to 40% of the initial conductance. Exposure of cell pairs to saline solutions saturated with CO2 (n = 15) or containing 2 mM halothane (n = 15) or 3.5 mM heptanol (n = 15) rapidly and reversibly reduced g(j). In eight cell pairs, gating of single junctional channels was observed during halothane-induced reduction in g(j). Most gating events at V-j < 40 mV were fit by a Gaussian distribution with a mean of similar to 100 pS, With V-j > 40 mV, smaller transitions of similar to 30 pS were also recorded, and the frequency and duration of the similar to 100-pS transitions decreased. Also, similar to 70-pS transitions between 30- and 100-pS conductances were observed in the absence of 70-pS transitions to or from the baseline, indicating that the 30-pS conductance was a substate induced by large V-j.