NESTED POLYMERASE CHAIN-REACTION FOR HIGH-SENSITIVITY DETECTION OF ENTEROVIRAL RNA IN BIOLOGICAL SAMPLES

被引：80

作者：

SEVERINI, GM

MESTRONI, L

FALASCHI, A

CAMERINI, F

GIACCA, M

机构：

[1] OSPED MAGGIORE TRIESTE,DEPT CARDIOL,I-34100 TRIESTE,ITALY

[2] UNIV TRIESTE,I-34100 TRIESTE,ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 05期

关键词：

D O I：

10.1128/JCM.31.5.1345-1349.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method based on nested polymerase chain reaction was developed for the detection of enteroviral genomes in biological samples. By taking advantage of the conserved 5' noncoding region of the enteroviral RNA, two sets of primers were utilized, enabling the detection either of a broad range of enteroviruses or of group B coxsackieviruses only. The sensitivity of the method is close to the detection of single molecules of viral RNA in as much as 1 mg of tissue sample. A preliminary study showed the usefulness of this technique for the analysis of endomyocardial biopsy samples from patients with idiopathic dilated cardiomyopathy and myocarditis.

引用

页码：1345 / 1349

页数：5

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