GONADOTROPIN HYPERSTIMULATION INFLUENCES THE S-35 METHIONINE METABOLISM OF MOUSE PREIMPLANTATION EMBRYOS

被引：5

作者：

WETZELS, AMM

ARTZ, MT

GOVERDE, HJM

BASTIAANS, BA

HAMILTON, CJCM

ROLLAND, R

机构：

[1] Department of Gynecology and Obstetrics, University Hospital Nijmegen, HB Nijmegen, 6500

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 10期

关键词：

MOUSE EMBRYOS; IN VIVO; GONADOTROPIN HYPERSTIMULATION; S-35 METHIONINE METABOLISM;

D O I：

10.1007/BF02212904

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The effects of gonadotropin stimulation on mouse embryo uptake and incorporation of S-35-methionine were studied. We found that the uptake of S-35-methionine was reduced in embryos of stimulated females in both the two-cell and the blastocyst developmental stage. The incorporation of S-35-methionine into protein was not statistically significantly different between the embryos of stimulated and those of unstimulated females. Qualitatively, protein synthesis was equal in both groups as determined with one-dimensional SDS-PAGE. The results are discussed and we conclude that mouse embryo viability in vivo is decreased by ovarian stimulation.

引用

页码：744 / 746

页数：3

共 11 条

[1]

Wetzels A.M.M., Punt-Van der Zalm A.P.E.M., Bastiaans L.A., Janssen B.A.P., Goverde H.J.M., Rolland R., The effects of human skin fibroblast monolayers on human sperm motility and mouse zygote development, Hum Reprod, 7, pp. 852-856, (1992)

[2]

Laemmli U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, pp. 680-685, (1970)

[3]

Holmberg S.R.M., Johnson M.H., Amino acid transport in the unfertilized and fertilized mouse egg, J Reprod Fert, 56, pp. 223-231, (1979)

[4]

Kaye P.L., Schultz G.A., Johnson M.H., Pratt H.P.M., Church R.B., Amino acid transport and exchange in preimplantation mouse embryos, J Reprod Fert, 65, pp. 367-380, (1982)

[5]

Brinster R.L., Uptake and incorporation of amino acids by the preimplantation mouse embryo, J Reprod Fert, 27, pp. 329-338, (1971)

[6]

Goddard M.J., Pratt H.P.M., Control of events during early cleavage of the mouse embryo: An analysis of the “2-cell block.”, J Embryol Exp Morphol, 73, pp. 111-133, (1983)

[7]

Epstein C.J., Smith S.A., Amino acid uptake and protein synthesis in preimplantation mouse embryos, Dev Biol, 33, pp. 171-184, (1973)

[8]

Van Blerkom J., Brockway G.O., Qualitative patterns of protein synthesis in the preimplantation mouse embryo. I. Normal pregnancy, Dev Biol, 44, pp. 148-157, (1975)

[9]

Elzamar M.M., Vogel R., Maternal factors influencing development of embryos from mice superovulated with gonadotropins, Reprod Toxicol, 2, pp. 135-138, (1989)

[10]

Vogel R., Spielman H., Genotoxic and embryotoxic effects of gonadotropin hyperstimulated ovulation of murine oocytes, preimplantation embryos, and term fetuses, Reprod Toxicol, 6, pp. 329-333, (1992)

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