NITRIC-OXIDE SYNTHASE IS LOCALIZED PREDOMINANTLY IN THE GOLGI-APPARATUS AND CYTOPLASMIC VESICLES OF VASCULAR ENDOTHELIAL-CELLS

被引:52
作者
OBRIEN, AJ [1 ]
YOUNG, HM [1 ]
POVEY, JM [1 ]
FURNESS, JB [1 ]
机构
[1] UNIV MELBOURNE,DEPT ANAT & CELL BIOL,PARKVILLE,VIC 3052,AUSTRALIA
来源
HISTOCHEMISTRY AND CELL BIOLOGY | 1995年 / 103卷 / 03期
关键词
D O I
10.1007/BF01454027
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The localization of nitric oxide synthase (NOS) in vascular endothelial cells of submucosal blood vessels from the guinea-pig ileum was examined using NADPH diaphorase histochemistry at the light microscopic level, and endothelial NOS immunohistochemistry at the light and electron microscopic level. The pattern of staining observed following NADPH diaphorase histochemistry and endothelial NOS immunohistochemistry was identical. Endothelial cells of the arterioles, capillaries and venules showed small patches of intense, perinuclear staining. Under the electron microscope, endothelial NOS immunoreactivity was found predominantly in association with the Golgi apparatus and with the membranes of some vesicles. Small regions of the plasma membrane and the rough endoplasmic reticulum also showed some immunoreactivity. The presence of NOS in the Golgi apparatus and in vesicles raises the possibility that NOS may be exteriorized by endothelial cells, and hence that nitric oxide is synthesized extracellularly.
引用
收藏
页码:221 / 225
页数:5
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