ASSAY AND CHARACTERIZATION OF DIURETIC FACTORS IN INSECTS

被引:31
作者
WHEELER, CH
COAST, GM
机构
[1] Department of Biology, Birkbeck College, University of London, London, WC1E 7HX England, Malet Street
关键词
bioassay; diuretic peptides; Fluid secretion; insects; Malpighian tubules; peptide isolation;
D O I
10.1016/0022-1910(90)90147-8
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Direct and indirect assays of diuresis in insects are reviewed and their advantages and disadvantages discussed in relation to their use during the isolation and characterisation of diuretic factors. The nature of known and putative diuretic peptides is contrasted and points of controversy over the identity of these peptides highlighted. The isolation and characterisation of a putative diuretic peptide from Acheta domesticus is discussed as a case study in the purification and characterisation of such peptides. © 1990.
引用
收藏
页码:23 / 34
页数:12
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