INDUCTION OF A HUMAN CARBONYL REDUCTASE GENE LOCATED ON CHROMOSOME-21

被引：51

作者：

FORREST, GL

AKMAN, S

KRUTZIK, S

PAXTON, RJ

SPARKES, RS

DOROSHOW, J

FELSTED, RL

GLOVER, CJ

MOHANDAS, T

BACHUR, NR

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV IMMUNOL,DUARTE,CA 91010

[2] CITY HOPE NATL MED CTR,DEPT MED ONCOL & THERAPEUT RES,DUARTE,CA 91010

[3] DIS DETECT INT,IRVINE,CA

[4] UNIV CALIF LOS ANGELES,MED CTR,DEPT MED,LOS ANGELES,CA 90024

[5] NCI,BIOL CHEM LAB,BETHESDA,MD 20205

[6] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT PEDIAT,TORRANCE,CA 90509

[7] UNIV MARYLAND,CTR CANC,BALTIMORE,MD 21201

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1048卷 / 2-3期

关键词：

cDNA; Daunorubicin reductase; Drug metabolism; MCF-7;

D O I：

10.1016/0167-4781(90)90050-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbonyl reductase (EC 1.1.1.184) belongs to the group of enzymes called aldo-keto reductases. It is a NADPH-dependent cytosolic protein with specificity for many carbonyl compounds including the antitumor anthracycline antibiotics, daunorubicin and doxorubicin. Human carbonyl reductase was cloned from a breast cancer cell line (MCF-7). The cDNA clone contained 1219 base paires with an open reading frame corresponding to 277 amino acids encoding a protein of Mr 30375. Southern analysis of genomic DNA digested with several restriction enzymes and analyzed by hybridization with a labeled cDNA probe indicated that carbonyl reductase is probably coded by a single gene and does not belong to a family of structurally similar enzymes. Southern analysis of 17 mouse / human somatic cell hybrids showed that carbonyl reductase is located on chromosome 21. Carbonyl reductase mRNA could be induced 3-4-fold in 24 h with 10 μM 2,(3)-t-butyl-4-hydroxyanisole (BHA), β-naphthoflavone or Sudan 1. © 1990.

引用

页码：149 / 155

页数：7

共 40 条

[1] HETEROGENEITY OF ANTHRACYCLINE ANTIBIOTIC CARBONYL REDUCTASES IN MAMMALIAN LIVERS
AHMED, NK
FELSTED, RL
BACHUR, NR
[J]. BIOCHEMICAL PHARMACOLOGY, 1978, 27 (23) : 2713 - 2719
[2] AHMED NK, 1979, J PHARMACOL EXP THER, V209, P12
[3] AKMAN SA, 1985, CANCER RES, V45, P5257
[4] AKMAN SA, 1987, J PHARMACOL EXP THER, V240, P486
[5] CELLULAR PHARMACODYNAMICS OF SEVERAL ANTHRACYCLINE ANTIBIOTICS
BACHUR, NR
STEELE, M
MERIWETHER, WD
HILDEBRAND, RC
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1976, 19 (05) : 651 - 654
[6] CYTOPLASMIC ALDO-KETO REDUCTASES - CLASS OF DRUG-METABOLIZING ENZYMES
BACHUR, NR
[J]. SCIENCE, 1976, 193 (4253) : 595 - 597
[7] INCREASE OF NAD(P)H-QUINONE REDUCTASE BY DIETARY ANTIOXIDANTS - POSSIBLE ROLE IN PROTECTION AGAINST CARCINOGENESIS AND TOXICITY
BENSON, AM
HUNKELER, MJ
TALALAY, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09): : 5216 - 5220
[8] BERENT S L, 1985, Biotechniques, V3, P208
[9] ERNSTER L, 1986, CHEM SCRIPTA A, V27, P1
[10] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13

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