PROTEIN PHOSPHATASE-2A FROM XENOPUS OOCYTES - CHARACTERIZATION DURING MEIOTIC CELL-DIVISION

被引：16

作者：

CORMIER, P ^{[1
]}

OSBORNE, HB ^{[1
]}

BASSEZ, T ^{[1
]}

POULHE, R ^{[1
]}

BELLE, R ^{[1
]}

MULNERLORILLON, O ^{[1
]}

机构：

[1] UNIV RENNES 1, BIOL & GENET DEV LAB, CNRS, URA 256, F-35042 RENNES, FRANCE

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

PROTEIN PHOSPHATASE-2A; ASSOCIATED PROTEIN; METAPHASE-SPECIFIC FORM; XENOPUS MEIOTIC CELL DIVISION;

D O I：

10.1016/0014-5793(91)81414-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polyclonal antibody was raised against bacterially produced catalytic alpha-subunit of protein phosphatase 2A (PP2AC) cloned from Xenopus ovarian library. The amount of PP2AC in Xenopus oocytes determined by Western blot analysis was 1 ng/mu-g of cytosolic protein. The antibody depleted PP2AC from oocyte extracts in association with 6 components (40, 62, 65, 80, 85 and 90 kDa). Prophase- and metaphase-arrested oocytes contained identical amounts of PP2AC. Metaphase oocytes showed one specific change in the 62 kDa protein associated with PP2AC.

引用

页码：185 / 188

页数：4

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