COPPER WIRE AMPEROMETRIC DETECTOR FOR CAPILLARY ELECTROPHORESIS

被引：37

作者：

ENGSTROMSILVERMAN, CE ^{[1
]}

EWING, AG ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT CHEM,DAVEY LAB 152,UNIVERSITY PK,PA 16802

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 02期

关键词：

CAPILLARY ELECTROPHORESIS; COPPER ELECTRODE DETECTION; AMINO ACIDS; CATECHOLAMINES; PEPTIDES;

D O I：

10.1002/mcs.1220030207

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of a copper wire amperometric detector for capillary electrophoresis is reported. Detection is accomplished by use of a porous glass joint, which allows amperometric detection at a copper wire electrode inserted in the end of the capillary. An anodic current is produced by a change in the copper oxide film solubility, resulting from complexation of copper ions with certain analytes at the electrode surface. This copper/copper oxide electrode has been used to detect nonelectroactive native amino acids and dipeptides. Subfemtomole detection limits in picoliter injection volumes have been obtained without solute derivatization. In addition, simultaneous analysis of nonelectroactive amino acids and electroactive catecholamines has been demonstrated.

引用

页码：141 / 145

页数：5

共 22 条

[1]

Jorgenson J.W., Lukacs K.D., Science, 222, (1983)

[2]

Gordon M.J., Huang X., Pentoney S.L., Zare R.N., Science, 242, (1988)

[3]

Cohen A.S., Karger B.L., High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins, Journal of Chromatography A, 397, (1987)

[4]

Ewing A.G., Wallingford R.A., Olefirowicz T.M., CAPILLARY ELECTROPHORESIS, Analytical Chemistry, 61, (1989)

[5]

Wallingford R.A., Ewing A.G., Anal. Chem., 60, (1988)

[6]

Olefirowicz T.M., Ewing A.G., Anal. Chem., 62, (1990)

[7]

Olefirowicz T.M., Ewing A.G., J. Neurosci. Meth., 34, (1990)

[8]

Cheng Y-F., Dovichi N.J., Science, 242, (1988)

[9]

Wallingford R.A., Ewing A.G., Anal. Chem., 60, (1988)

[10]

Wallingford R.A., Ewing A.G., Anal. Chem., 61, (1989)

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