OPTIMUM BETA-D-GLUCOSIDASE SUPPLEMENTATION OF CELLULASE FOR EFFICIENT CONVERSION OF CELLULOSE TO GLUCOSE

To assess optimal saccharification performance, alpha-cellulose and dilute acid pretreated aspen (DAA) wood meal were subjected to various loadings of commercial cellulase and beta-D-glucosidase preparations. Fifteen international filter paper units (IFPU)/g cellulose content and 30 IFPU/g cellulose content were required to digest 95% of the available cellulose in alpha-cellulose and pretreated aspen, respectively. The optimal supplementation ratios, based on Genencor GC 123 cellulase and beta-D-glucosidase from Novo SP188 for the alpha-cellulose and DAA digestions range from 0.25 to 0.5 and 0.12 to 0.25, respectively.