EXTENSION OF THE APPLICATION OF THE THERMAL IN-SITU REACTION ON NH2 LAYERS TO THE DETECTION OF CATECHOLAMINES IN BIOLOGICAL-MATERIALS

被引：18

作者：

KLAUS, R ^{[1
]}

FISCHER, W ^{[1
]}

HAUCK, HE ^{[1
]}

机构：

[1] E MERCK AG,D-64271 DARMSTADT,GERMANY

来源：

CHROMATOGRAPHIA | 1993年 / 37卷 / 3-4期

关键词：

THIN-LAYER CHROMATOGRAPHY; NH2-SILICA GEL; CATECHOLAMINES; URINE SAMPLES;

D O I：

10.1007/BF02275850

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes a thin-layer chromatographic method for the determination of catecholamines in human urine. Chromatographic separation on NH2-modified silica gel layers is followed by in-situ visualization brought about by heating the plate. The use of this derivatization technique for other urine components, such as creatine, creatinine, uric acid and glucose, is also discussed.

引用

收藏

页码：133 / 143

页数：11

相关论文

共 38 条

[1]

ANDERSON GM, 1988, J CHROMATOGR, V424, P473

[2] ASSESSMENT OF SEMI-AUTOMATED CATECHOLAMINE ASSAY BY HPLC - CHOICE OF REVERSE PHASE C18 OR CATION-EXCHANGE COLUMNS [J].

BESCHI, M ;

CASTELLANO, M ;

AGABITIROSEI, E ;

RIZZONI, D ;

ROSSINI, P ;

MUIESAN, G .

CHROMATOGRAPHIA, 1987, 24 :455-459

[3] DANSYLATION OF AMINES, PHENOLIC AND CATECHOLIC AMINES AND AMINO-ACIDS IN APROTIC-SOLVENTS [J].

DAVIS, BA .

JOURNAL OF CHROMATOGRAPHY, 1978, 151 (02) :252-255

[4] DETERMINATION OF CATECHOLAMINES IN URINE (AND PLASMA) BY LIQUID-CHROMATOGRAPHY AFTER ONLINE SAMPLE PRETREATMENT ON SMALL ALUMINA OR DIHYDROXYBORYLSILICA COLUMNS [J].

DEJONG, J ;

POINT, AJF ;

TJADEN, UR ;

BEEKSMA, S ;

KRAAK, JC .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 414 (02) :285-300

[5] SELECTIVE PRECONCENTRATION ON A METAL LOADED SILICA PRECOLUM AND ONLINE HPLC SEPARATION OF CATECHOL DERIVATIVES IN A COMPLEX MEDIUM - APPLICATION TO THE ANALYSIS OF CATECHOLAMINES IN URINE [J].

DESCOMBES, AA ;

HAERDI, W .

CHROMATOGRAPHIA, 1989, 28 (9-10) :459-464

[6] QUANTITATIVE ANALYSIS OF CATECHOLAMINE AND SEROTONIN METABOLITES BY QUENCHING FLUORESENCE ON THIN LAYER CHROMATOGRAPHIC PLATE [J].

DWORZAK, E ;

HUCK, H .

JOURNAL OF CHROMATOGRAPHY, 1971, 61 (01) :162-&

[7]

GEISSLER HE, 1976, ARZNEIMITTEL-FORSCH, V26, P75

[8] MODIFIED SAMPLE PREPARATION FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ELECTROCHEMICAL ASSAY OF URINARY CATECHOLAMINES [J].

GOLDSTEIN, DS .

JOURNAL OF CHROMATOGRAPHY, 1983, 275 (01) :174-177

[9] MICRO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SYSTEM WITH MICRO PRECOLUMN AND DUAL ELECTROCHEMICAL DETECTOR FOR DIRECT INJECTION-ANALYSIS OF CATECHOLAMINES IN BODY-FLUIDS [J].

GOTO, M ;

NAKAMURA, T ;

ISHII, D .

JOURNAL OF CHROMATOGRAPHY, 1981, 226 (01) :33-42

[10] FULL AUTOMATION OF CATECHOLAMINE METABOLITE DETERMINATION BY COLUMN SWITCHING AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

GROSSI, G ;

BARGOSSI, A ;

BATTISTONI, R ;

LIPPI, A ;

SPROVIERI, G .

JOURNAL OF CHROMATOGRAPHY, 1989, 465 (01) :113-119

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