ELECTRON-MICROSCOPIC INVESTIGATIONS ON FREESTANDING MIXED LIPID LANGMUIR-BLODGETT-KUHN MONOLAYERS - PHASE-SEPARATION AND AGING PROCESS

被引:5
作者
LIESER, G [1 ]
MITTLERNEHER, S [1 ]
SPINKE, J [1 ]
KNOLL, W [1 ]
机构
[1] MAX PLANCK INST POLYMER RES,D-55021 MAINZ,GERMANY
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1192卷 / 01期
关键词
LANGMUIR-BLODGETT-KUHN MONOLAYER; PHASE SEPARATION; AGING; LIPID MONOLAYER; CALCIUM ION; EELS; ELECTRON MICROSCOPY;
D O I
10.1016/0005-2736(94)90137-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipid monolayers were prepared by the Langmuir-Blodgett-Kuhn technique (LBK) as free-standing films spanning a diameter of up to 1 mu m. These films were investigated by electron spectroscopic imaging (ESI) and electron energy less spectroscopy (EELS). The Gee-standing monolayer is shown to be in a transient state in which an aging process is proceeding: after storage for two weeks in air at room temperature the films tear off the edges of the perforated supporting film, Ca2+ ions induced lateral phase separation in these films prepared from a 50:50 mixture of lecithin/glycerol could be visualized by means of ESI, i.e., by comparing micrographs below and above the Ca absorption edge in the EEL spectrum. The domain sizes of the demixed phases were determined to vary between 30 and 60 nm. In addition it was shown that the counter ion of the negatively charged glycerol in these films is Ca2+ and not Na+.
引用
收藏
页码:14 / 20
页数:7
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