IDENTIFICATION OF A CDNA CLONE SPECIFIC FOR THE OLIGODENDROCYTE-DERIVED REPULSIVE EXTRACELLULAR-MATRIX MOLECULE J1-160/180

被引：20

作者：

FUSS, B

POTT, U

FISCHER, P

SCHWAB, ME

SCHACHNER, M

机构：

[1] SWISS FED INST TECHNOL, DEPT NEUROBIOL, CH-8093 ZURICH, SWITZERLAND

[2] UNIV ZURICH, BRAIN RES INST, CH-8006 ZURICH, SWITZERLAND

[3] UNIV HEIDELBERG, ZENTRUM MOLEC BIOL HEIDELBERG, W-6900 HEIDELBERG, GERMANY

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1991年 / 29卷 / 03期

关键词：

CELL REPULSION; CENTRAL NERVOUS SYSTEM; J1-160/180; OLIGODENDROCYTE; RECOGNITION MOLECULE;

D O I：

10.1002/jnr.490290305

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A cDNA clone specific for the oligodendrocyte-derived extracellular matrix glycoproteins J1-160/180 was obtained from a lambda-ZAPII expression library using polyclonal antibodies generated against mouse J1-160. The library was constructed from poly(A)+-RNA isolated from O1 antigen-positive rat oligodendrocytes. The cDNA clone expressed a fusion protein that was recognized by the J1-160/180-specific monoclonal antibodies 596, 619, and 620, and, weakly, 597. The fusion protein was not recognized by polyclonal antibodies to mouse J1/tenascin. The cDNA clone with an insert of approximately 5.6 kb in size contained the nucleotide sequence coding for the amino acid sequence of the N-terminus of a tryptic peptide derived from mouse J1-160. The developmental and tissue distribution of the mRNA recognized by the cDNA clone is in agreement with the described expression of the J1-160/180 proteins.

引用

页码：299 / 307

页数：9

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