MASS-SPECTROMETRY OF PROTEINS - STUDIES OF PARVALBUMINS BY PLASMA DESORPTION, LASER DESORPTION AND ELECTROSPRAY MASS-SPECTROMETRY

被引：10

作者：

ROEPSTORFF, P ^{[1
]}

KLARSKOV, K ^{[1
]}

ANDERSEN, J ^{[1
]}

MANN, M ^{[1
]}

VORM, O ^{[1
]}

ETIENNE, G ^{[1
]}

PARELLO, J ^{[1
]}

机构：

[1] FAC PHARM MONTPELLIER,CNRS,UNITE RECH 1111,F-34060 MONTPELLIER,FRANCE

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES | 1991年 / 111卷

关键词：

D O I：

10.1016/0168-1176(91)85053-O

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

A family of closely related proteins in the 11-12 kDa range, parvalbumins, were investigated by plasma desorption, laser desorption and electrospray mass spectrometry. The last two techniques were found to be advantageous in terms of sensitivity, precision and tolerance of impurities and contaminants. Electrospray mass spectrometry had the further advantage that minor components present in the sample were detected. The data obtained indicated a sequence error in one parvalbumin, confirmed N-terminal acetylation in others and in general allow screening for possible candidates for the phylogenetically interesting "elongated" parvalbumins.

引用

页码：151 / 172

页数：22

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