SYNCHROTRON X-RAY STUDIES OF LIPIDS AND MEMBRANES - A CRITIQUE

被引:31
作者
CUNNINGHAM, BA
BRAS, W
LIS, LJ
QUINN, PJ
机构
[1] SERC,DARESBURY LAB,NETHERLANDS ORG SCI RES,WARRINGTON WA4 4AD,CHESHIRE,ENGLAND
[2] UNIV LONDON KINGS COLL,DEPT BIOCHEM,LONDON W8 7AH,ENGLAND
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1994年 / 29卷 / 02期
基金
英国惠康基金;
关键词
SYNCHROTRON X-RADIATION; LIPID DISPERSION; PHASE TRANSITION; TRANSITION KINETICS; X-RAY DIFFRACTION;
D O I
10.1016/0165-022X(94)90046-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This review gives a description of techniques, suitable for the study of lipid dispersions and unorientated membranes, that are available at synchrotron facilities to determine either the kinetics of transitional phenomena in the time after a temperature or pressure jump is initiated, or the phases present while a sample undergoes a phase transition. Included in this description is information about synchrotron X-ray sources, sample holders and temperature controllers, detection systems, as well as data reduction. Examples involving lipid dispersions are provided to illustrate the application of these methods using synchrotron radiation.
引用
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页码:87 / 111
页数:25
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