DETERMINATION OF UREA IN URINE USING A CONDUCTIVITY CELL WITH SURFACE-ACOUSTIC-WAVE RESONATOR-BASED MEASUREMENT CIRCUIT

被引：25

作者：

CHEN, K ^{[1
]}

LIU, DZ ^{[1
]}

NIE, LH ^{[1
]}

YAO, SZ ^{[1
]}

机构：

[1] HUNAN UNIV,DEPT CHEM & CHEM ENGN,CHANGSHA 410082,PEOPLES R CHINA

来源：

TALANTA | 1994年 / 41卷 / 12期

关键词：

D O I：

10.1016/0039-9140(94)00197-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A conductivity cell employing a 61 MHz surface acoustic wave resonator-based measurement circuit was applied to the detection of the urea/urease reaction. The kinetic enzymatic parameters of the urease were estimated from the frequency shifts. The effects of pH, temperature and inhibitor on the response of the enzyme conductivity measurement system were investigated. The system was applied to rapid determination of urea in small urine samples. The lowest detection limit of urea was 30 ng/ml.

引用

页码：2195 / 2200

页数：6

共 19 条

[1] BIOSENSING WITH COATED-WIRE ELECTRODES .2. UREA SENSORS [J].

ABDULLA, HM ;

GREENWAY, GM ;

PLATT, AE ;

FIELDEN, PR .

ANALYST, 1989, 114 (12) :1575-1578

[2] CONDUCTIMETRIC ASSAY OF A BACTERIAL LIPASE, USING TRIACETIN AS A SUBSTRATE [J].

BALLOT, C ;

FAVREBONVIN, G ;

WALLACH, JM .

ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1982, 15 (13) :1119-1129

[3] SUITABLE POTENTIOMETRIC ENZYME SENSORS FOR UREA AND CREATININE [J].

CAMPANELLA, L ;

SAMMARTINO, MP ;

TOMASSETTI, M .

ANALYST, 1990, 115 (06) :827-830

[4] MULTIANALYTE MINIATURE CONDUCTANCE BIOSENSOR [J].

CULLEN, DC ;

SETHI, RS ;

LOWE, CR .

ANALYTICA CHIMICA ACTA, 1990, 231 (01) :33-40

[5] A CONDUCTOMETRIC DEVICE FOR MONITORING OF ENZYME-CATALYZED REACTIONS [J].

DUFFY, P ;

MEALET, C ;

WALLACH, JM ;

FOMBON, JJ .

ANALYTICA CHIMICA ACTA, 1988, 211 (1-2) :205-211

[6] A CONDUCTOMETRIC METHOD FOR THE ASSAY OF AMIDASE AND PEPTIDASE ACTIVITIES [J].

HILL, CR ;

TOMALIN, G .

ANALYTICAL BIOCHEMISTRY, 1982, 120 (01) :165-175

[7]

HOLME DJ, 1983, ANAL BIOCHEM, P245

[8] THERMOCHEMICAL STUDY OF HYDROLYSIS OF UREA BY UREASE [J].

JESPERSEN, ND .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1975, 97 (07) :1662-1667

[9] SOME PROPERTIES AND PURIFICATION OF UREASE [J].

LYNN, KR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 146 (01) :205-+

[10]

LYNN KR, 1964, BIOCHIM BIOPHYS ACTA, V81, P333

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