MOLECULAR RELATIONSHIP BETWEEN THE A-CHROMOSOME AND B-CHROMOSOME OF CREPIS-CAPILLARIS

被引：11

作者：

JAMILENA, M ^{[1
]}

GARRIDORAMOS, M ^{[1
]}

REJON, CR ^{[1
]}

REJON, MR ^{[1
]}

机构：

[1] UNIV GRANADA,FAC CIENCIAS,DEPT GENET,E-18071 GRANADA,SPAIN

来源：

HEREDITY | 1994年 / 73卷

关键词：

B CHROMOSOMES; CREPIS CAPILLARIS; F-PERT; MICROSATELLITES;

D O I：

10.1038/hdy.1994.151

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

The-molecular structure of the B chromosome of Crepis capillaris has been analysed by DNA comparisons of plants with 2B and 0B chromosomes. No additional band, and no differences in the band intensity were detected in 2B plants after digestion with 18 restriction enzymes. One hundred and five clones were obtained from 2B plants using the phenol-emulsion reassociation technique in formamide (F-PERT). Only one (clone pCc21) gave rise to additional hybridization bands in the DNA from 2B plants, indicating that this sequence occurs both in A and B chromosomes. Clone pCc21 contains a 54-bp microsatellite sequence rich in CA dinucleotides, similar to those found in other eukaryotic genomes. We conclude that the B chromosome of C. capillaris carries no specific highly repetitive sequence, but consists of sequences also present in the A chromosomes. The possibility remains that the heterochromatic pericentromeric region of the B has a different organization with respect to standard heterochromatin.

引用

页码：527 / 531

页数：5

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