STUDY ON THE FLUORESCENCE SYSTEM OF TB-ATP-PHEN FOR THE DETERMINATION OF ATP

被引:6
作者
YANG, JH
ZHOU, GJ
JIE, NQ
GAO, ZQ
ZOU, HB
YOU, CP
HAN, Y
机构
[1] Department of Chemistry, Shandong University, Jinan, Shandong
关键词
ATP; TERBIUM(III); SPECTROFLUOROMETRY; ATP DETERMINATION;
D O I
10.1080/00032719408007244
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
It was found that ATP could form a ternary complex with Tb3+ and Phen, which can emit the characteristic fluorescence of Tb3+. The experiment showed that the fluorescence intensity of the system was maxmium in the following conditions: pH of the solution is 7.2, the concentrations of Tb3+ and phenanthroline (Phen) are 2.0X10(-5)mol.L(-1) and 2,0X10(-4)mol.L(-1), respectively. At the optimum conditions, a linear relationship is obtained between the fluorescence intensity and ATP concentration in the range of 1.0x10(-6)-1.0.X10(-5)mol.L(-1). Traces of ATP in drugs (ATP disodium salt tablets) can be determined using the standard addition method. The luminescence mechanism of the system was discussed.
引用
收藏
页码:2181 / 2193
页数:13
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