GENES FOR COMPONENTS OF THE CHLOROPLAST TRANSLATIONAL APPARATUS ARE CONSERVED IN THE REDUCED 73-KB PLASTID DNA OF THE NONPHOTOSYNTHETIC EUGLENOID FLAGELLATE ASTASIA-LONGA

被引：25

作者：

GOCKEL, G ^{[1
]}

HACHTEL, W ^{[1
]}

BAIER, S ^{[1
]}

FLISS, C ^{[1
]}

HENKE, M ^{[1
]}

机构：

[1] UNIV BONN, INST BOT, D-53115 BONN, GERMANY

来源：

CURRENT GENETICS | 1994年 / 26卷 / 03期

关键词：

ASTASIA LONGA; PLASTID DNA; RIBOSOMAL PROTEIN GENES; TRANSFER-RNA GENES;

D O I：

10.1007/BF00309557

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The colourless, nonphotosynthetic protist Astasia sia longa is phylogenetically related to Euglena gracilis. The 73-kb plastid DNA (ptDNA) of A. longa is about half the size of most chloroplast DNAs (cpDNAs). More than 38 kb of the Astasia ptDNA sequence has been determined. No genes for photosynthetic function have been found except for rbcL. Identified genes include rpoB, tufA, and genes coding for three rRNAs, 17 tRNAs, and 13 ribosomal proteins. Not only is the nucleotide sequence of these genes highly conserved between A. longa and E. gracilis, but a number of these genes are clustered in a similar fashion and have introns in the same positions in both species. The results further support the idea that photosynthetic genes normally encoded in cpDNA have been preferentially lost in Astasia, but that the chloroplast genes coding for components of the plastid translational apparatus have been maintained. This apparatus might be needed for the expression of rbcL and also for that of still unidentified nonphotosynthetic genes of Astasia ptDNA.

引用

页码：256 / 262

页数：7

共 37 条

[1] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA
CHEN, EY
SEEBURG, PH
[J]. DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02): : 165 - 170
[2] EUGLENA-GRACILIS CHLOROPLAST RIBOSOMAL-PROTEIN OPERON - A NEW CHLOROPLAST GENE FOR RIBOSOMAL-PROTEIN L5 AND DESCRIPTION OF A NOVEL ORGANELLE INTRON CATEGORY DESIGNATED GROUP-III
CHRISTOPHER, DA
HALLICK, RB
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (19) : 7591 - 7608
[3] ORGANIZATION OF RIBOSOMAL-PROTEIN GENES RPL23, RPL2, RPS19, RPL22 AND RPS3 ON THE EUGLENA-GRACILIS CHLOROPLAST GENOME
CHRISTOPHER, DA
CUSHMAN, JC
PRICE, CA
HALLICK, RB
[J]. CURRENT GENETICS, 1988, 14 (03) : 275 - 285
[4] CHRISTOPHER DA, 1990, PLANT CELL, V2, P659
[5] LOSS OF PHOTOSYNTHETIC AND CHLORORESPIRATORY GENES FROM THE PLASTID GENOME OF A PARASITIC FLOWERING PLANT
DEPAMPHILIS, CW
PALMER, JD
[J]. NATURE, 1990, 348 (6299) : 337 - 339
[6] INVIVO TRANSCRIPTIONAL PRODUCTS OF THE CHLOROPLAST DNA OF EUGLENA-GRACILIS
DIX, KP
RAWSON, JRY
[J]. CURRENT GENETICS, 1983, 7 (04) : 265 - 272
[7] STRUCTURE AND ORGANIZATION OF MARCHANTIA-POLYMORPHA CHLOROPLAST GENOME .3. GENE ORGANIZATION OF THE LARGE SINGLE COPY REGION FROM RBCL TO TRNI(CAU)
FUKUZAWA, H
KOHCHI, T
SANO, T
SHIRAI, H
UMESONO, K
INOKUCHI, H
OZEKI, H
OHYAMA, K
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (02) : 333 - 351
[8] GINGRICH JC, 1985, J BIOL CHEM, V260, P6156
[9] SEQUENCING OF 16S-23S SPACER IN A RIBOSOMAL-RNA OPERON OF EUGLENA-GRACILIS CHLOROPLAST DNA REVEALS 2 TRANSFER-RNA GENES
GRAF, L
KOSSEL, H
STUTZ, E
[J]. NATURE, 1980, 286 (5776) : 908 - 910
[10] COMPLETE SEQUENCE OF EUGLENA-GRACILIS CHLOROPLAST DNA
HALLICK, RB
HONG, L
DRAGER, RG
FAVREAU, MR
MONFORT, A
ORSAT, B
SPIELMANN, A
STUTZ, E
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (15) : 3537 - 3544

← 1 2 3 4 →