RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF CLINICALLY AND ENVIRONMENTALLY ISOLATED CRYPTOCOCCUS-NEOFORMANS IN NAGASAKI

We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var, neoformans (serotype A) in the southern Japanese prefecture of Nagasaki, The random amplified polymorphic DNA profiles obtained by using three primers revealed six patterns among 21 clinical isolates and three patterns among 8 environmental isolates, Pattern I was the most common (18 of 29 isolates) and was found among isolates obtained throughout the entire Nagasaki Prefecture, Patterns I, III, and IV were found among both clinical and environmental isolates. Patterns I and IV had a characteristic distribution, and in particular, pattern IV was isolated exclusively (five of six isolates) from isolates from Nagasaki City, Two environmental isolates from two locations associated strongly with two patients revealed identical random amplified polymorphic DNA patterns (patterns I and IV) for isolates from each patient. Our results suggest that clinical and environmental isolates belong to the same pool of C., neoformans isolates and that these isolates have certain geographic locations, although the number of isolated strains was limited.