SYNOVIAL-FLUIDS FROM INFECTED JOINTS CONTAIN METALLOPROTEINASE - TISSUE INHIBITOR OF METALLOPROTEINASE (TIMP) COMPLEXES

被引：13

作者：

CAWSTON, T

MCLAUGHLAN, P

COUGHLAN, R

KYLE, V

HAZLEMAN, B

机构：

[1] Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1033卷 / 01期

基金：

英国医学研究理事会;

关键词：

Collagenase; Metalloproteinase; Proteinase inhibitor complex; Synovial fluid; Tissue inhibitor; α[!sub]2[!/sub]-Macroglobulin;

D O I：

10.1016/0304-4165(90)90200-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Samples of synovial fluids aspirated from patients with septic arthritis prior to the commencement of any treatment contained active metalloproteinases but proteinase no inhibitory activity. We therefore assayed these samples for proteinase-inhibitor complexes. Although no biologically active α2-macroglobulin or tissue inhibitor of metalloproteinase (TIMP) was present in the fluids, immunoassay of the samples clearly showed that high molecular weight proteinase-TIMP complexes were present. It is proposed that high levels of active metalloproteinases are released from neutrophils into septic synovial fluids and that these proteinases complex all the available TIMP, forming metalloproteinase-TIMP complexes. © 1990.

引用

页码：96 / 102

页数：7

共 25 条

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