FRAME-SHIFT MUTATIONS WITHIN THE VACCINIA VIRUS A-TYPE INCLUSION PROTEIN GENE

被引:42
作者
AMEGADZIE, BY [1 ]
SISLER, JR [1 ]
MOSS, B [1 ]
机构
[1] NIAID,VIRAL DIS LAB,BLDG 4,ROOM 229,BETHESDA,MD 20892
关键词
D O I
10.1016/0042-6822(92)90046-R
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic basis for the failure of vaccinia virus (strain WR) to forma full-length 150 kiloDalton (kDa) A-type inclusion protein was determined by sequencing a 4.1-kb pair segment of DNA and analyzing its transcription products. Open reading frames predicted to encode slightly overlapping 84.5- and 27.1-kDa proteins homologous to contiguous N-terminal segments of the A-type inclusion protein of cowpox virus were found. A putative deletion of two adjacent nucleotides occurring within several consecutive AG repeats and an insertion of 8 nucleotides accounted for the first and second reading frame shifts, respectively. Additional small mutations affecting reading frames were present in the C-terminal region of the gene. The vaccinia and cowpox virus mRNAs encoding the disparate size A-type inclusion proteins were similar in length, had equivalent 5′ and 3′ ends, and were expressed late in infection indicating the absence of mutations affecting transcriptional signals. © 1992.
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收藏
页码:777 / 782
页数:6
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