STRUCTURE AND EVOLUTION OF A FAMILY OF GENES ENCODING ANTISEPTIC AND DISINFECTANT RESISTANCE IN STAPHYLOCOCCUS-AUREUS

被引：129

作者：

LITTLEJOHN, TG ^{[1
]}

DIBERARDINO, D ^{[1
]}

MESSEROTTI, LJ ^{[1
]}

SPIERS, SJ ^{[1
]}

SKURRAY, RA ^{[1
]}

机构：

[1] MONASH UNIV, DEPT MICROBIOL, CLAYTON, VIC 3168, AUSTRALIA

来源：

GENE | 1991年 / 101卷 / 01期

基金：

英国医学研究理事会;

关键词：

RECOMBINANT DNA; ETHIDIUM BROMIDE; PLASMID EVOLUTION; MEMBRANE PROTEIN; TRANSPORT;

D O I：

10.1016/0378-1119(91)90224-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Resistance to antiseptics and disinfectants in Staphylococcus aureus, encoded by the qacC/qacD gene family, is associated with genetically dissimilar small, nontransmissible (pSK89) and large conjugative (pSK41) plasmids. The qacC and qacD genes were analysed in detail through deletion mapping and nucleotide sequence analysis, and shown to encode the same polypeptide, predicted to be 107 aa in size. Direct repeat elements flank the qacD gene, elements which also flank the qacC gene in truncated forms. These elements contain palA sequences, regions of DNA required for replication of some plasmids in S. aureus. The qacC gene is predicted to have evolved from the qacD gene, and in the process to have become reliant on new promoter sequences for its expression. The entire sequence of the 2.4-kb plasmid pSK89 (which contains qacC) was determined, and is compared with other plasmids from Gram+ bacteria.

引用

页码：59 / 66

页数：8

共 52 条

[1] COMPLETE NUCLEOTIDE-SEQUENCE OF PTZ12, A CHLORAMPHENICOL-RESISTANCE PLASMID OF BACILLUS-SUBTILIS [J].

AOKI, T ;

NOGUCHI, N ;

SASATSU, M ;

KONO, M .

GENE, 1987, 51 (01) :107-111

[2] STRUCTURAL ORGANIZATION OF PLP1, A CRYPTIC PLASMID FROM LACTOBACILLUS-PLANTARUM CCM-1904 [J].

BOUIA, A ;

BRINGEL, F ;

FREY, L ;

KAMMERER, B ;

BELARBI, A ;

GUYONVARCH, A ;

HUBERT, JC .

PLASMID, 1989, 22 (03) :185-192

[3] THE USE OF SYNTHETIC OLIGONUCLEOTIDES WITH UNIVERSAL TEMPLATES FOR RAPID DNA SEQUENCING - RESULTS WITH STAPHYLOCOCCAL REPLICON PC221 [J].

BRENNER, DG ;

SHAW, WV .

EMBO JOURNAL, 1985, 4 (02) :561-568

[4]

BRISSONNOEL A, 1988, J BIOL CHEM, V263, P15880

[5] NUCLEOTIDE-SEQUENCE OF GENE LINA ENCODING RESISTANCE TO LINCOSAMIDES IN STAPHYLOCOCCUS-HAEMOLYTICUS [J].

BRISSONNOEL, A ;

COURVALIN, P .

GENE, 1986, 43 (03) :247-253

[6] MOLECULAR ANALYSIS OF A GENTAMICIN RESISTANCE TRANSPOSON-LIKE ELEMENT ON PLASMIDS ISOLATED FROM NORTH-AMERICAN STAPHYLOCOCCUS-AUREUS STRAINS [J].

BYRNE, ME ;

GILLESPIE, MT ;

SKURRAY, RA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (11) :2106-2113

[7] NUCLEOTIDE-SEQUENCE ANALYSIS OF IS256 FROM THE STAPHYLOCOCCUS-AUREUS GENTAMICIN-TOBRAMYCIN-KANAMYCIN-RESISTANCE TRANSPOSON TN4001 [J].

BYRNE, ME ;

ROUCH, DA ;

SKURRAY, RA .

GENE, 1989, 81 (02) :361-367

[8]

BYRNE ME, 1990, MOL BIOL STAPHYLOCOC, P165

[9] NUCLEOTIDE-SEQUENCE AND COMPARATIVE-ANALYSIS OF THE FRD OPERON ENCODING THE FUMARATE REDUCTASE OF PROTEUS-VULGARIS - EXTENSIVE SEQUENCE DIVERGENCE OF THE MEMBRANE ANCHORS AND ABSENCE OF AN FRD-LINKED AMPC CEPHALOSPORINASE GENE [J].

COLE, ST .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 167 (03) :481-488

[10] THE GENBANK-SERVER AT THE UNIVERSITY-OF-HOUSTON [J].

DAVISON, DB ;

CHAPPELEAR, JE .

NUCLEIC ACIDS RESEARCH, 1990, 18 (06) :1571-1572

← 1 2 3 4 5 6 →