PRESSURE EFFECTS ON SPECTRA OF PHOTOSYNTHETIC LIGHT-HARVESTING PIGMENT-PROTEIN COMPLEXES

被引：40

作者：

FREIBERG, A

ELLERVEE, A

KUKK, P

LAISAAR, A

TARS, M

TIMPMANN, K

机构：

[1] Institute of Physics, Estonian Academy of Sciences, 2400 Tartu

来源：

CHEMICAL PHYSICS LETTERS | 1993年 / 214卷 / 01期

关键词：

D O I：

10.1016/0009-2614(93)85447-V

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The influence of high (up to 9 kbar) hydrostatic pressure on the absorption and fluorescence emission spectra of photosynthetic light-harvesting pigment-protein complexes isolated from purple bacteria Rhodospirillum rubrum has been studied at room temperature and at 77 K. Under pressure at room temperature all spectral bands exhibit a red-shift at a rate of between 30 and 120 cm-1/kbar for different bands. From these pressure shifts the compressibility of the protein matrix can be estimated. The compressibility is remarkably different for the protein surrounding bacteriochlorophyll a molecules (kappa greater-than-or-equal-to 25 +/- 5 Mbar-1) than for the one surrounding spirilloxanthin molecules (kappa = 10 +/- 2 Mbar-1). This indicates that the elastic properties of a protein are locally specific.

引用

收藏

页码：10 / 16

页数：7

相关论文

共 21 条

[1] ABSORPTION SPECTRAL SHIFTS OF CAROTENOIDS RELATED TO MEDIUM POLARIZABILITY [J].

ANDERSSON, PO ;

GILLBRO, T ;

FERGUSON, L ;

COGDELL, RJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1991, 54 (03) :353-360

[2]

ANDERSSON PO, COMMUNICATION

[3] STRUCTURE, FUNCTION AND ORGANIZATION OF ANTENNA POLYPEPTIDES AND ANTENNA COMPLEXES FROM THE 3 FAMILIES OF RHODOSPIRILLANEAE [J].

BRUNISHOLZ, RA ;

ZUBER, H .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 15 (1-2) :113-140

[4]

BUJNOWSKI W, 1973, INSTRUM EXP TECH, V16, P274

[5] EFFECTS OF HIGH-PRESSURE ON PHOTOCHEMICAL REACTION CENTERS FROM RHODOPSEUDOMONAS-SPHEROIDES [J].

CLAYTON, RK ;

DEVAULT, D .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1972, 15 (02) :165-&

[6] SPECTRAL HOLE BURNING AT HIGH HYDROSTATIC-PRESSURE [J].

ELLERVEE, A ;

JAANISO, R ;

KIKAS, J ;

LAISAAR, A ;

SUISALU, A ;

SHCHERBAKOV, V .

CHEMICAL PHYSICS LETTERS, 1991, 176 (05) :472-476

[7] PRESSURE AND LOW-TEMPERATURE EFFECTS ON THE FLUORESCENCE EMISSION-SPECTRA AND LIFETIMES OF THE PHOTOSYNTHETIC COMPONENTS OF CYANOBACTERIA [J].

FOGUEL, D ;

CHALOUB, RM ;

SILVA, JL ;

CROFTS, AR ;

WEBER, G .

BIOPHYSICAL JOURNAL, 1992, 63 (06) :1613-1622

[8] PROTEINS AND PRESSURE [J].

FRAUENFELDER, H ;

ALBERDING, NA ;

ANSARI, A ;

BRAUNSTEIN, D ;

COWEN, BR ;

HONG, MK ;

IBEN, IET ;

JOHNSON, JB ;

LUCK, S ;

MARDEN, MC ;

MOURANT, JR ;

ORMOS, P ;

REINISCH, L ;

SCHOLL, R ;

SCHULTE, A ;

SHYAMSUNDER, E ;

SORENSEN, LB ;

STEINBACH, PJ ;

XIE, AH ;

YOUNG, RD ;

YUE, KT .

JOURNAL OF PHYSICAL CHEMISTRY, 1990, 94 (03) :1024-1037

[9] PICOSECOND FLUORESCENCE SPECTROSCOPY OF LIGHT-HARVESTING ANTENNA COMPLEXES FROM RHODOSPIRILLUM-RUBRUM IN THE 300-4-K TEMPERATURE-RANGE - COMPARISON WITH THE DATA ON CHROMATOPHORES [J].

FREIBERG, A ;

TIMPMANN, K .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 15 (1-2) :151-158

[10] COMPRESSIBILITY OF GLOBULAR-PROTEINS IN WATER AT 25-DEGREES-C [J].

GEKKO, K ;

NOGUCHI, H .

JOURNAL OF PHYSICAL CHEMISTRY, 1979, 83 (21) :2706-2714