CALDESMON, CALPONIN AND ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN SUBCULTURED SMOOTH-MUSCLE CELLS FROM HUMAN AIRWAYS

被引:31
作者
DURANDARCZYNSKA, W [1 ]
MARMY, N [1 ]
DURAND, J [1 ]
机构
[1] INST PHYSIOL,CH-1700 FRIBOURG,SWITZERLAND
关键词
D O I
10.1007/BF00267827
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Calponin and caldesmon are two proteins considered to play a regulatory role in smooth muscle contraction, which have never previously been found to be expressed in subcultured cells. In the present study, immunocytochemistry and immunoblotting were performed to identify these proteins in smooth muscle cells (SMC) from human bronchi. It was found that human airway SMC, kept in a non-proliferative state, continued to express caldesmon and calponin at least until the 8th passage. The expression of alpha-smooth muscle actin studied under the same conditions was also shown to be preserved in subcultured bronchial SMC.
引用
收藏
页码:465 / 471
页数:7
相关论文
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