HEPARIN-INDUCED THROMBOCYTOPENIA - AN IMPROVED METHOD OF DETECTION BASED ON LUMI-AGGREGOMETRY

被引:49
作者
STEWART, MW
ETCHES, WS
BOSHKOV, LK
GORDON, PA
机构
[1] Department of Laboratory Medicine and Pathology, University of Alberta Hospitals, Edmonton, Alberta
关键词
HEPARIN; THROMBOCYTOPENIA; PLATELET; LUMINOGRAPHY; AGGREGOMETRY;
D O I
10.1111/j.1365-2141.1995.tb05265.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Heparin-induced thrombocytopenia (HIT) is a recognized complication of heparin administration, Early detection of this syndrome is essential in the prevention of immune-mediated thromboembolic sequelae. The C-14-serotonin release assay (SRA) has been used in reference laboratories to identify sera from patients on heparin therapy capable of inducing platelet dense granule release. In an attempt to improve existing methodologies, we employed luminographic detection of platelet-dense granule ATP release as an endpoint of HIT antibody-mediated platelet activation, Sera tested included 10 SRA confirmed positive and five SRA confirmed negative samples (to establish the assay), five samples from patients with thrombocytopenia not on heparin therapy and 34 patients suspected of HIT syndrome. All SRA confirmed positive sera (n = 19) were positive by the luminographic procedure, 24/26 SRA confirmed negative sera and five sera from thrombocytopenic patients not on heparin therapy were negative using luminography. Two of four sera yielding equivocal SRA results were found to be positive by the luminographic technique, The data suggest that the use of a luminaggregometer in the coagulation laboratory to detect HIT antibody-induced platelet activation is a reliable alternative to the SRA. The luminographic procedure is both rapid and sensitive, and does not require the use of biohazardous radio-isotopes.
引用
收藏
页码:173 / 177
页数:5
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