PULSED UV LASER-INDUCED FLUORESCENCE DETECTION OF NATIVE PEPTIDES AND PROTEINS IN CAPILLARY ELECTROPHORESIS

被引：54

作者：

CHAN, KC

JANINI, GM

MUSCHIK, GM

ISSAQ, HJ

机构：

[1] Program Resources, Inc./DynCrop, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, P.O. Box B

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 9-10期

关键词：

D O I：

10.1080/10826079308019903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pulsed UV laser operating at 248 nm was used for the laser-induced fluorescence (LIF) detection of native tryptophan-containing compounds in capillary electrophoresis. The limit of detection (LOD) of tryptophan was found to be 3.3x10(-9) M (S/N=2). The LODs of the model proteins conalbumin and bovine serum albumin were found to be 1.3x10(-9) and 4x10(-9) M (S/N=2), respectively. These results were at least two orders of magnitude more sensitive when compared to UV absorption at 214 nm.

引用

页码：1877 / 1890

页数：14

共 14 条

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