SEQUENTIAL TREATMENT BY PHOSPHOTUNGSTIC ACID AND URANYL ACETATE ENHANCES THE ADHERENCE OF LIPID-MEMBRANES AND MEMBRANE-PROTEINS TO HYDROPHOBIC EM GRIDS

被引：4

作者：

BARNAKOV, AN

机构：

[1] Biophysics Department, Washington State University, Pullman, Washington

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1994年 / 175卷

关键词：

GRID SPECIMEN INTERACTION; LIPID MEMBRANES; MEMBRANE PROTEIN;

D O I：

10.1111/j.1365-2818.1994.tb03481.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A simple procedure for screening by electron microscopic observations of conditions for the reconstitution of membrane proteins into lipid bilayers is described. This procedure consists of a 5-10-s treatment of electron microscopic grids, to which the sample has already been applied, with 1% phosphotungstic acid before proceeding with final staining in uranyl acetate. The method substantially enhances the adherence of lipid membranes and membrane protein particles to hydrophobic collodion/ carbon grids.

引用

页码：171 / 174

页数：4

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