DIAGNOSIS OF ENTEROVIRAL MENINGITIS BY USING PCR WITH A COLORIMETRIC MICROWELL DETECTION ASSAY

被引：161

作者：

ROTBART, HA

SAWYER, MH

FAST, S

LEWINSKI, C

MURPHY, N

KEYSER, EF

SPADORO, J

KAO, SY

LOEFFELHOLZ, M

机构：

[1] UNIV CALIF SAN DIEGO,DEPT PEDIAT,SAN DIEGO,CA 92103

[2] ROCHE MOLEC SYST,BRANCHBURG,NJ

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 10期

关键词：

D O I：

10.1128/JCM.32.10.2590-2592.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylase. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

引用

收藏

页码：2590 / 2592

页数：3

相关论文

共 13 条

[1] ASEPTIC-MENINGITIS IN INFANTS LESS-THAN-2 YEARS OF AGE - DIAGNOSIS AND ETIOLOGY [J].

BERLIN, LE ;

RORABAUGH, ML ;

HELDRICH, F ;

ROBERTS, K ;

DORAN, T ;

MODLIN, JF .

JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (04) :888-892

[2]

CHONMAITREE T, 1982, JAMA-J AM MED ASSOC, V247, P1843, DOI 10.1001/jama.247.13.1843

[3]

HSIUNG GD, 1973, DIAGNOSTIC VIROLOGY, P54

[4] DETECTION OF CHLAMYDIA-TRACHOMATIS IN ENDOCERVICAL SPECIMENS BY POLYMERASE CHAIN-REACTION [J].

LOEFFELHOLZ, MJ ;

LEWINSKI, CA ;

SILVER, SR ;

PUROHIT, AP ;

HERMAN, SA ;

BUONAGURIO, DA ;

DRAGON, EA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) :2847-2851

[5] USE OF URACIL DNA GLYCOSYLASE TO CONTROL CARRY-OVER CONTAMINATION IN POLYMERASE CHAIN-REACTIONS [J].

LONGO, MC ;

BERNINGER, MS ;

HARTLEY, JL .

GENE, 1990, 93 (01) :125-128

[6] DETECTION AND DIFFERENTIATION OF PICORNAVIRUSES IN CLINICAL-SAMPLES FOLLOWING GENOMIC AMPLIFICATION [J].

OLIVE, DM ;

ALMUFTI, S ;

ALMULLA, W ;

KHAN, MA ;

PASCA, A ;

STANWAY, G ;

ALNAKIB, W .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :2141-2147

[7] DIAGNOSIS OF ENTEROVIRAL MENINGITIS WITH THE POLYMERASE CHAIN-REACTION [J].

ROTBART, HA .

JOURNAL OF PEDIATRICS, 1990, 117 (01) :85-89

[8] ENZYMATIC RNA AMPLIFICATION OF THE ENTEROVIRUSES [J].

ROTBART, HA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :438-442

[9]

SAWYER MH, 1994, J PEDIATR INFECT DIS, V13, P177

[10] TEMPORAL AND GEOGRAPHIC PATTERNS OF ISOLATES OF NONPOLIO ENTEROVIRUS IN THE UNITED-STATES, 1970-1983 [J].

STRIKAS, RA ;

ANDERSON, LJ ;

PARKER, RA .

JOURNAL OF INFECTIOUS DISEASES, 1986, 153 (02) :346-351